PMID: 9530880Apr 8, 1998Paper

Compensation of decreased triose phosphate/phosphate translocator activity by accelerated starch turnover and glucose transport in transgenic tobacco

Planta
R E HäuslerU I Flügge

Abstract

Tobacco (Nicotiana tabacum L.) plants were transformed with an antisense construct of the chloroplast triose phosphate/phosphate translocator (TPT). Three transformant lines of the T4 progeny, which showed a large decrease in the transcript level of the TPT were used for further biochemical and physiological characterisation. In all antisense lines tested, TPT transport activity was diminished by 50-70% compared with the wild type (WT). Despite this high reduction in the transport capacity, alpha TPT plants lacked any visible phenotype. Hexokinase and alpha-amylase activities were increased in alpha TPT plants compared with the WT, whereas activities of ribulose-1,5-bisphosphate carboxylase/oxygenase and ADP-glucose pyrophosphorylase (AGPase) were not affected. At the end of a 14-h light period, leaf starch contents in alpha TPT lines were similar to those of the WT and controls, indicating that a decrease in the TPT had no effect on starch accumulation. Sucrose contents were diminished by more than 50% in alpha TPT lines compared with control plants. The time course of starch accumulation revealed a transient increase in the starch content in a selected alpha TPT line after 6 h in the light, followed by a decrease towards the ...Continue Reading

Citations

Mar 6, 1999·Current Opinion in Plant Biology·U I Flügge
Mar 30, 2004·Journal of Experimental Botany·Andreas P M WeberLars M Voll
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Marie UmberLéon Otten
Jun 18, 2002·Plant Physiology·Samuel C ZeemanAlison M Smith
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Ulf-Ingo Flugge
May 3, 2005·Annual Review of Plant Biology·Alison M SmithSteven M Smith
Jun 21, 2011·BMC Systems Biology·Ambarish NagChristopher H Chang
Jan 22, 2011·PLoS Genetics·Young-Hee Cho, Sang-Dong Yoo
Feb 14, 2014·Journal of Experimental Botany·Jessica SchmitzRainer E Häusler
Aug 11, 2015·The Plant Journal : for Cell and Molecular Biology·Patrik M KarlssonCornelia Spetea
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Mark StittBjörn Usadel
Dec 30, 2009·Molecular Plant·Nicole Linka, Andreas P M Weber
Aug 1, 2004·The New Phytologist·Samuel C ZeemanAlison M Smith
Dec 10, 1998·Plant Physiology·J SchleucherT D Sharkey

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