Compensatory increases in tear volume and mucin levels associated with meibomian gland dysfunction caused by stearoyl-CoA desaturase-1 deficiency

Scientific Reports
Takaaki InabaKazuo Tsubota

Abstract

The stearoyl-CoA desaturase (SCD) family of enzymes catalyzes monounsaturated fatty acid synthesis by inserting a cis double bond at the Δ9 position of saturated fatty acids. Disruption of these enzymes has been reported to induce a severe dry skin phenotype. Since lipid abnormalities in the meibomian glands have been associated with dry eye, we analyzed selected eye tissues contributing to tear volume and composition in genetically SCD-1-deficient mice (SCD-1 KO), including the lacrimal glands and conjunctiva. Previous histopathological analysis had revealed atrophy and loss of meibomian glands; taken together with the increased goblet cell and MUC5AC expression in the conjunctiva reported here, these findings suggest that the tear volume and mucin levels secreted are enhanced in the absence of lipid secretion as a compensatory mechanism. The expression of lipid metabolism genes in lacrimal glands was decreased in SCD1 KO mice. Thus, these results provide new pathophysiological mechanisms to pursue with regard to meibomian gland dysfunction. In addition, lack of SCD-1 causes a compensatory increase in the tear volume and mucin levels associated with changes in expression of lipid metabolism genes. These results may be useful a...Continue Reading

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Citations

Apr 3, 2019·Veterinary Ophthalmology·Rosélia L S AraújoPaula D Galera
May 23, 2020·Experimental Biology and Medicine·Masaya HiraishiYasuhiro Kon
Dec 11, 2019·International Journal of Molecular Sciences·Georgi As GeorgievNorihiko Yokoi
Nov 22, 2018·Analytical and Bioanalytical Chemistry·Anis Barmada, Scott A Shippy
Jul 22, 2019·Experimental Eye Research·Céline PortalJean-Luc Desseyn
Apr 16, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chae Eun KimJaewook Yang
Nov 28, 2021·Indian Journal of Ophthalmology·Samrat Chatterjee, Deepshikha Agrawal

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Methods Mentioned

BETA
light microscopy
PCR
electrophoresis

Software Mentioned

ImageJ

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