Compensatory mechanisms associated with the hyperdynamic function of phospholamban-deficient mouse hearts

Circulation Research
G ChuEvangelia G Kranias

Abstract

Phospholamban ablation is associated with significant increases in the sarcoplasmic reticulum Ca(2+)-ATPase activity and the basal cardiac contractile parameters. To determine whether the observed phenotype is due to loss of phospholamban alone or to accompanying compensatory mechanisms, hearts from phospholamban-deficient and age-matched wild-type mice were characterized in parallel. There were no morphological alterations detected at the light microscope level. Assessment of the protein levels of the cardiac sarcoplasmic reticulum Ca(2+)-ATPase, calsequestrin, myosin, actin, troponin I, and troponin T revealed no significant differences between phospholamban-deficient and wild-type hearts. However, the ryanodine receptor protein levels were significantly decreased (25%) upon ablation of phospholamban, probably in an attempt to regulate the release of Ca2+ from the sarcoplasmic reticulum, which had a significantly higher diastolic Ca2+ content in phospholamban-deficient compared with wild-type hearts (16.0 +/- 2.2 versus 8.6 +/- 1.0 mmol Ca2+/kg dry wt, respectively). The increases in Ca2+ content were specific to junctional sarcoplasmic reticulum stores, as there were no alterations in the Ca2+ content of the mitochondria or ...Continue Reading

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Citations

Feb 12, 2004·Journal of Molecular and Cellular Cardiology·Kimberly N GregoryEvangelia G Kranias
Oct 31, 2008·Molecular Biology of the Cell·Elizabeth VafiadakiEvangelia G Kranias
Sep 14, 2010·Cardiovascular Research·Mayilvahanan ShanmugamGopal J Babu
Dec 22, 1999·Annals of the New York Academy of Sciences·G ChuE G Kranias
Jun 9, 2000·Annual Review of Physiology·H Kiriazis, E G Kranias
Jan 8, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Ashwin AkkiRobert G Weiss
Mar 18, 2003·The Journal of Clinical Investigation·Qiujing SongEvangelia G Kranias
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J HajjarA Rosenzweig
Jul 5, 2001·Journal of Molecular and Cellular Cardiology·R DashE G Kranias
Sep 2, 1999·Journal of Molecular and Cellular Cardiology·A G BrittsanE G Kranias
Nov 6, 1998·Magma·M SpindlerJ S Ingwall
Feb 1, 2012·Journal of Molecular and Cellular Cardiology·Jason H Yang, Jeffrey J Saucerman
Mar 28, 2007·Heart Failure Clinics·Jason R Waggoner, Evangelia G Kranias
Mar 28, 2007·Heart Failure Clinics·Djamel LebecheRoger J Hajjar
Jun 18, 2004·Annals of the New York Academy of Sciences·Martin Morad, My Chau
May 3, 2008·Journal of Cellular Biochemistry·Ilka PinzJoanne S Ingwall
Sep 8, 2004·Journal of Molecular and Cellular Cardiology·Joanne S Ingwall
Jan 24, 2007·Journal of Molecular Biology·Elizabeth VafiadakiAikaterini Kontrogianni-Konstantopoulos
Apr 1, 2004·The Surgical Clinics of North America·Babar B ChaudhriRoger J Hajjar
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Sep 4, 2003·The Journal of Clinical Investigation·Maryam M JavadpourJoanne S Ingwall
Jan 3, 2019·American Journal of Physiology. Endocrinology and Metabolism·Daniel GamuA Russell Tupling
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·David H MacLennan, Evangelia G Kranias
Feb 18, 2004·Nature Medicine·Julian C BrazJeffery D Molkentin
Dec 5, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Darrell D BelkeWolfgang H Dillmann
Dec 29, 2000·Annals of Medicine·K Frank, E G Kranias
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Sep 12, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ilka PinzJoanne S Ingwall

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