Compensatory periplasmic nitrate reductase activity supports anaerobic growth of Pseudomonas aeruginosa PAO1 in the absence of membrane nitrate reductase.

Canadian Journal of Microbiology
Nadine E Van AlstConstantine G Haidaris

Abstract

Nitrate serves as a terminal electron acceptor under anaerobic conditions in Pseudomonas aeruginosa. Reduction of nitrate to nitrite generates a transmembrane proton motive force allowing ATP synthesis and anaerobic growth. The inner membrane-bound nitrate reductase NarGHI is encoded within the narK1K2GHJI operon, and the periplasmic nitrate reductase NapAB is encoded within the napEFDABC operon. The roles of the 2 dissimilatory nitrate reductases in anaerobic growth, and the regulation of their expressions, were examined by use of a set of deletion mutants in P. aeruginosa PAO1. NarGHI mutants were unable to grow anaerobically, but plate cultures remained viable up to 120 h. In contrast, the nitrate sensor-response regulator mutant DeltanarXL displayed growth arrest initially, but resumed growth after 72 h and reached the early stationary phase in liquid culture after 120 h. Genetic, transcriptional, and biochemical studies demonstrated that anaerobic growth recovery by the NarXL mutant was the result of NapAB periplasmic nitrate reductase expression. A novel transcriptional start site for napEFDABC expression was identified in the NarXL mutant grown anaerobically. Furthermore, mutagenesis of a consensus NarL-binding site mono...Continue Reading

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Citations

Nov 22, 2011·Antioxidants & Redox Signaling·Emilio BuenoMaria J Delgado
Dec 9, 2017·Frontiers in Microbiology·Muralidhar TataUdo Bläsi
May 24, 2017·Infection and Immunity·Gilberto Hideo KaihamiRegina Lúcia Baldini
Feb 24, 2016·Journal of Bacteriology·José Manuel Borrero-de AcuñaDieter Jahn
Aug 31, 2014·Applied and Environmental Microbiology·Victoria G PederickChristopher A McDevitt
Feb 22, 2018·Journal of Bacteriology·Yu-Cheng LinLars E P Dietrich
May 25, 2021·Frontiers in Molecular Biosciences·Sylvain Durand, Maude Guillier

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