Compensatory rebalancing of rice prolamins by production of recombinant prolamin/bioactive peptide fusion proteins within ER-derived protein bodies

Plant Cell Reports
Fumio TakaiwaKenjiro Ozawa

Abstract

Bioactive peptide was produced by fusion to rice prolamins in transgenic rice seeds. Their accumulation levels were affected by their deposition sites and by compensatory rebalancing between prolamins within PB-Is. Peptide immunotherapy using analogue peptide ligands (APLs) is one of promising treatments against autoimmune diseases. Use of seed storage protein as a fusion carrier is reasonable strategy for production of such small size bioactive peptides. In this study, to examine the efficacy of various rice prolamins deposited in ER-derived protein bodies (PB-Is), the APL12 from the Glucose-6-phosphate isomerase (GPI325-339) was expressed by fusion to four types of representative prolamins under the control of the individual native promoters. When the 14 and 16 kDa Cys-rich prolamins, which were localized in middle layer of PB-Is, were used for production of the APL12, they highly accumulated in transgenic rice seeds (~ 200 µg/grain). By contrast, fusion to the 10 and 13 kDa prolamins, which were localized in the core and outermost layer of PB-Is, resulted in lower levels of accumulation (~ 40 µg/grain). These results suggest that accumulation levels were highly affected by their deposition sites. Next, when different prolami...Continue Reading

References

Jan 1, 1996·Annual Review of Immunology·J Sloan-Lancaster, P M Allen
Mar 30, 1999·Nature Biotechnology·F GotoF Takaiwa
Apr 16, 2005·Current Opinion in Biotechnology·Eva StogerPaul Christou
Nov 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hidenori TakagiFumio Takaiwa
Apr 15, 2006·Plant & Cell Physiology·Hiroshi YasudaFumio Takaiwa
Dec 1, 2006·Plant Biotechnology Journal·Yoshifumi TadaFumio Takaiwa
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Hidenori TakagiFumio Takaiwa
Jan 9, 2007·Plant Biotechnology Journal·Fumio TakaiwaYuhya Wakasa
Jun 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tomonori NochiHiroshi Kiyono
Aug 13, 2008·Plant Biotechnology Journal·Monica A Schmidt, Eliot M Herman
Mar 25, 2010·Plant Biotechnology Journal·Youko OonoFumio Takaiwa
Nov 13, 2010·Bioscience, Biotechnology, and Biochemistry·Masaharu KurodaChikako Mikami
Jan 13, 2011·The Plant Journal : for Cell and Molecular Biology·Yuhya WakasaFumio Takaiwa
Apr 28, 2011·Plant & Cell Physiology·Ai NagamineToshihiro Kumamaru
Feb 14, 2012·Plant Foods for Human Nutrition·Chibuike C Udenigwe, Rotimi E Aluko
Oct 5, 2013·Biotechnology Journal·Yuhya Wakasa, Fumio Takaiwa
Jan 15, 2014·Plant Cell Reports·Jiquan OuDaichnag Yang
Jun 24, 2015·Plant Biotechnology Journal·Fumio TakaiwaTakachika Hiroi
Sep 2, 2016·Plant Cell Reports·Fumio TakaiwaKenjiro Ozawa
Aug 19, 2017·Plant Science : an International Journal of Experimental Plant Biology·Fumio TakaiwaTaiji Kawakatsu

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoresis
X-ray

Software Mentioned

NIH Image J

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.