PMID: 8955279Dec 1, 1996Paper

Competition between functional signal peptides demonstrates variation in affinity for the secretion pathway

Journal of Bacteriology
H ChenD A Kendall

Abstract

We have developed a system for examining the relative affinity of two different signal peptides for the protein secretion pathway in Escherichia coli. This system involves the expression of a modified alkaline phosphatase which possesses two signal peptides arranged in tandem. When both signal peptides have the wild-type sequence, cleavage after the first and cleavage after the second occur with nearly equal frequency. In both cases the remainder of the protein is transported to the periplasm. Thus both signal peptides effectively compete with each other for entrance to the secretion pathway. When the hydrophobicity of the second signal peptide is altered by small increments, we find that the more hydrophobic signal peptide is preferentially utilized. Thus, a more hydrophobic signal peptide can outcompete even an efficient wild-type signal sequence. The crossover point, for utilization of the second to the first signal peptide, is marked and occurs over a very small change in hydrophobicity. Our results suggest that the small differences in the hydrophobicity of wild-type signal peptides may have critical consequences: preproteins with the more hydrophobic signals could dominate one pathway, leaving those with only slightly les...Continue Reading

References

Oct 4, 1991·Cell·B C Hann, P Walter
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M SakaguchiT Omura
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J AkimaruS Mizushima
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D B OliverG P Jarosik
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y AudigierG Blobel
Jul 1, 1988·European Journal of Biochemistry·G von Heijne, Y Gavel
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E A EvansG Blobel
Jul 1, 1985·Journal of Bacteriology·C A Kumamoto, J Beckwith
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Watanabe, G Blobel
Jun 9, 1995·The Journal of Biological Chemistry·H Chen, D A Kendall
Sep 1, 1994·Molecular Microbiology·J W Izard, D A Kendall
Jan 1, 1994·Annual Review of Cell Biology·P Walter, A E Johnson
Jun 1, 1994·Journal of Cellular Biochemistry·S L RuschD A Kendall
Jan 1, 1994·Molecular Microbiology·J Luirink, B Dobberstein
Jul 1, 1996·The Journal of Cell Biology·D T NgP Walter

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Citations

Mar 27, 2013·Applied Microbiology and Biotechnology·Kheng Oon LowRosli Md Illias
Aug 29, 2001·FEMS Microbiology Reviews·K H van WelyA J Driessen
Apr 16, 2008·Infection and Immunity·Jason W RoschMichael G Caparon
Mar 28, 2019·Journal of Molecular Biology·Eric Lindner, Stephen H White
May 3, 2014·Biochemistry·Andy J WoworJames L Cole

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