Competition of aminoacyl-tRNA synthetases for tRNA ensures the accuracy of aminoacylation.

Nucleic Acids Research
J M ShermanD Söll

Abstract

The accuracy of protein biosynthesis rests on the high fidelity with which aminoacyl-tRNA synthetases discriminate between tRNAs. Correct aminoacylation depends not only on identity elements (nucleotides in certain positions) in tRNA (1), but also on competition between different synthetases for a given tRNA (2). Here we describe in vivo and in vitro experiments which demonstrate how variations in the levels of synthetases and tRNA affect the accuracy of aminoacylation. We show in vivo that concurrent overexpression of Escherichia coli tyrosyl-tRNA synthetase abolishes misacylation of supF tRNA(Tyr) with glutamine in vivo by overproduced glutaminyl-tRNA synthetase. In an in vitro competition assay, we have confirmed that the overproduction mischarging phenomenon observed in vivo is due to competition between the synthetases at the level of aminoacylation. Likewise, we have been able to examine the role competition plays in the identity of a non-suppressor tRNA of ambiguous identity, tRNA(Glu). Finally, with this assay, we show that the identity of a tRNA and the accuracy with which it is recognized depend on the relative affinities of the synthetases for the tRNA. The in vitro competition assay represents a general method of ob...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L Pallanck, L H Schulman
May 8, 1990·Biochemistry·R ChattapadhyayL H Schulman
Aug 1, 1990·Biochimie·H Bedouelle
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W H McClainJ Schneider
Dec 11, 1990·Nucleic Acids Research·H HimenoM Shimizu
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·U Varshney, U L RajBhandary
Jan 1, 1989·Annual Review of Biochemistry·J Normanly, J Abelson
Feb 2, 1989·Nature·C Francklyn, P Schimmel
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M J Rogers, D Söll
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R Sampson, O C Uhlenbeck
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D M CrothersG Söll
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H InokuchiD Söll

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Citations

Jan 1, 1993·Biochimie·H BedouelleR Nageotte
Aug 27, 2013·Analytical Biochemistry·Akimitsu KugimiyaDaiki Funamoto
Jun 1, 1994·Journal of Biomolecular Structure & Dynamics·D Bhattacharyya, M Bansal
Feb 6, 2017·Biochimica Et Biophysica Acta. General Subjects·Kyle S HoffmanChristopher J Brandl
Dec 21, 1993·Biochemistry·M FrugierR Giegé
Mar 25, 2009·FEMS Microbiology Letters·Sonja Lesjak, Ivana Weygand-Durasevic

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