Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells.

Journal of Biomedicine & Biotechnology
Alicja JankowskaElzbieta Grzesiuk

Abstract

Competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract. In this study, we examined the ability of Lactobacillus paracasei to inhibit the adhesion of pathogenic Salmonella enterica to human colon adenocarcinoma Caco-2 cells. Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively. In adhesion experiments, bacteria were added to the cells for 2 or 4 hours. The number of attached bacteria was expressed as colony-forming units (CFUs), Caco-2 cells were counted in hematocytometer. Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L. paracasei and almost 27-fold higher after 4 hours of contact. Two types of experiments were done: coincubation (both bacteria were added to Caco-2 cells simultaneously), and preincubation (L. paracasei was incubated with Caco-2 cells first, and then S. enterica was added). In coincubation experiment, the presence of L. paracasei decreased S. enterica adhesion by 4-fold and in...Continue Reading

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Citations

May 14, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M KatouliR Möllby
Nov 9, 2012·Folia Microbiologica·Kristýna TurkováBojana Bogovič Matijašić
Sep 17, 2013·The Journal of Microbiology·Li-Qun WangXiang-Chen Meng
Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Shengbin RaoFumiki Morimatsu
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Apr 29, 2015·Journal of Dairy Science·Xin WangTingtao Chen
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Nov 9, 2016·Frontiers in Cellular and Infection Microbiology·Xue GaoQinghua Yu
Mar 1, 2012·World Journal of Gastrointestinal Pathophysiology·Linda Chia-Hui YuYen-Hsuan Ni
Mar 13, 2016·Applied and Environmental Microbiology·Kayla R ArendtA Elizabeth Arnold
Feb 13, 2013·The British Journal of Nutrition·Luis FontanaAngel Gil
Jul 31, 2019·Applied Microbiology and Biotechnology·Anna Koryszewska-BagińskaTamara Aleksandrzak-Piekarczyk
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Apr 18, 2009·Inflammatory Bowel Diseases·Luca PrisciandaroGordon Howarth
Oct 29, 2021·Journal of Applied Microbiology·Abdul RaheemGuangzhi Zhang

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