Abstract
Twenty-three phage-displayed peptides that specifically bind to an anti-benzothiostrobin monoclonal antibody (mAb) in the absence or presence of benzothiostrobin were isolated from a cyclic 8-residue peptide phage library. Competitive and noncompetitive phage enzyme linked immunosorbent assays (ELISAs) for benzothiostrobin were developed by using a clone C3-3 specific to the benzothiostrobin-free mAb and a clone N6-18 specific to the benzothiostrobin immunocomplex, respectively. Under the optimal conditions, the half maximal inhibition concentration (IC50) of the competitive phage ELISA and the concentration of analyte producing 50% saturation of the signal (SC50) of the noncompetitive phage ELISA for benzothiostrobin were 0.94 and 2.27 ng mL(-1), respectively. The noncompetitive phage ELISA showed higher selectivity compared to the competitive. Recoveries of the competitive and the noncompetitive phage ELISAs for benzothiostrobin in cucumber, tomato, pear and rice samples were 67.6-119.6% and 70.4-125.0%, respectively. The amounts of benzothiostrobin in the containing incurred residues samples detected by the two types of phage ELISAs were significantly correlated with that detected by high-performance liquid chromatography (H...Continue Reading
References
Nov 24, 1999·Chemical Research in Toxicology·G ShanB D Hammock
Apr 12, 2001·Journal of Applied Microbiology·K Thirumala-DeviM A Mayo
Jun 30, 2005·Environmental Science & Technology·Soledad CardozoGualberto G González-Sapienza
Sep 12, 2007·Analytical Chemistry·A González-TecheraG González-Sapienza
Nov 2, 2007·Analytical Chemistry·A González-TecheraG González-Sapienza
Apr 9, 2008·Bioconjugate Chemistry·A González-TecheraG González-Sapienza
Apr 16, 2008·Environmental Science & Technology·Hee-Joo KimBruce D Hammock
Dec 23, 2008·Analytical Biochemistry·Hee-Joo KimBruce D Hammock
May 21, 2009·Analytical Biochemistry·Junko InabaYoshihito Suzuki
Jun 23, 2009·Analytical Biochemistry·Wen-Jun GuiGuo-Nian Zhu
Feb 16, 2010·Analytical Biochemistry·Hee-Joo KimBruce D Hammock
May 4, 2010·Analytica Chimica Acta·Jingwei SunShuo Wang
Oct 5, 2010·Analytical Chemistry·M A RossottiG González-Sapienza
Dec 15, 2010·Analytical Chemistry·Hee-Joo KimBruce D Hammock
Feb 12, 2013·Journal of Agricultural and Food Chemistry·Yanru WangBruce D Hammock
Oct 8, 2013·Analytical and Bioanalytical Chemistry·Jia WangTing Xu
Oct 14, 2014·Pesticide Biochemistry and Physiology·Congying XuMingguo Zhou
Citations
Nov 14, 2016·Talanta·Anna KolosovaBoris B Dzantiev
Nov 28, 2018·Analytical and Bioanalytical Chemistry·He ChenBruce D Hammock
Feb 11, 2020·Frontiers in Microbiology·Xianfeng RenPeiwu Li
Feb 3, 2021·Journal of Hazardous Materials·Hui-Jun FuBruce Hammock
May 18, 2021·Food Chemistry·He ChenXiude Hua
Jul 15, 2021·Analytical Chemistry·Álvaro Luque-UríaMaría C Moreno-Bondi
Nov 6, 2018·Analytical Chemistry·Yuan DingMinghua Wang
Dec 28, 2017·Analytical Chemistry·Yuan DingMinghua Wang
Aug 23, 2019·ACS Applied Materials & Interfaces·He ChenXiude Hua
Sep 30, 2021·Mikrochimica Acta·He ChenXiude Hua