Competitive electrochemical immunosensor for maduramicin detection by multiple signal amplification strategy via hemin@Fe-MIL-88NH2 /AuPt

Biosensors & Bioelectronics
Mei HuGaiping Zhang

Abstract

Maduramicin (MD) is a type of monoglycoside polyether ionophore antibiotic that can effectively treat coccidiosis and facilitate animal growth. However, its extensive and excessive use brings potential risk to human health. Herein, an electrochemical immunosensor based on indirect competitive format was fabricated for analysis of MD residue in eggs by a multiple signal amplification system. Initially, Au nanoparticles were deposited onto glassy carbon electrode surface to load the coating antigen MD-BSA and to improve conductivity. Then the signal amplification platform was constructed by encapsulating hemin into Fe-MIL-88 NH2 metal-organic frameworks (hemin@MOFs), and then the obtained composites were decorated with AuPt nanoparticles. The synthesized hemin@MOFs/AuPt was not only used as a signal amplification mediator, but also utilized as a carrier for immobilization of horseradish peroxidase-conjugated affinipure goat anti-mouse antibody (Ab2-HRP) and horseradish peroxidase (HRP). The constructed hemin@MOFs/AuPt-Ab2-HRP bioconjugates could effectively amplify the current signal since hemin@MOFs, AuPt and HRP all exhibited high catalytic activity towards the hydrogen peroxide. Moreover, the established immunosensor showed hi...Continue Reading

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Citations

Apr 10, 2020·Biotechnology Journal·Liping DuChunsheng Wu
Jul 30, 2020·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Linlin ChenGaiping Zhang
Jan 26, 2021·Biosensors & Bioelectronics·Chi ZhangHongliang Han
Sep 8, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yan LengJiayu Wan
Aug 24, 2021·Journal of Biomedical Research·Jiarong ZhangJin Chen
Nov 30, 2021·Analytical Methods : Advancing Methods and Applications·Shouhui ChenYonghai Song

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