Competitive electrochemical platform for ultrasensitive cytosensing of liver cancer cells by using nanotetrahedra structure with rolling circle amplification

Biosensors & Bioelectronics
Duanping SunZuanguang Chen

Abstract

In this work, a competitive and label-free electrochemical platform was performed for the ultrasensitive cytosensing of liver cancer cells based on DNA nanotetrahedron (NTH) structure and rolling circle amplification (RCA) directed DNAzyme strategy. The multifunctional nanoprobes were fabricated through a DNA primer probe, carboxyfluorescein (FAM) functionalized TLS11a aptamer and horseradish peroxidase (HRP) immobilized on the surfaces of the platinum nanoparticles (PtNPs). Then the NTH-based complementary DNA (cDNA) probe, complementary to the TLS11a aptamer, was attached on a disposable screen-printed gold electrode (SPGE) for increasing the reactivity and accessibility with the prepared nanoprobes. Due to the primer probe and the circular probe with G-quadruplex sequences for RCA, it can lead to the formation of numerous G-quadruplex/hemin DNAzyme, thus generating a remarkable electrochemical response. When the target cells were present, the nanoprobes were released from the SPGE due to the specific recognition of TLS11a aptamers for HepG2 cells, resulting in the electrochemical signal changes. The cytosensor was ultrasensitive for HepG2 tumor cell detection with a detection limit of 3 cell per mL. Furthermore, this strateg...Continue Reading

Citations

Feb 27, 2020·Thoracic Cancer·Zhenhua ZhangMin Liu
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
Apr 21, 2021·Journal of Hazardous Materials·Baozhong ZhangBaoshan He
May 24, 2021·Analytical Biochemistry·Rozi Asadi, Hamidreza Mollasalehi
Apr 20, 2021·Annual Review of Biomedical Engineering·Lia A StanciuNoor Mohammad
Aug 8, 2021·International Journal of Molecular Sciences·Han ZhouZhenguo Zhang

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