PMID: 6406642Jan 1, 1983Paper

Competitive enzyme immunoassay for apolipoprotein A-II

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
B DufauxG Assmann

Abstract

A competitive enzyme immunoassay for apolipoprotein A-II was developed. Microtitre plates were used as a solid phase and coated with anti-apolipoprotein A-II antibodies. Purified apolipoprotein A-II, labelled with horseradish peroxidase was used as competing ligand. The assay was examined with respect to the optimal amounts of specific anti-apolipoprotein A-II antibodies and apolipoprotein A-II-enzyme conjugate. The displacement curves showed a good parallelism between serum and purified apolipoprotein A-II. Delipidation of serum did not affect the content of apolipoprotein A-II. Cross-reactivity with apolipoprotein A-I was minimal. The intra- and inter-assay coefficients of variation in the medium range were 9.0 and 11.8% respectively. The assay might be well-suited for clinical routine.

References

Jun 1, 1976·Metabolism: Clinical and Experimental·J J AlbersJ J Hoover
Mar 1, 1977·The Journal of Clinical Investigation·G AssmannK Oette
Jul 1, 1977·The Journal of Clinical Investigation·M C Cheung, J J Albers
Jan 1, 1979·Journal of Immunological Methods·S M BurtL J Kricka
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.