Competitive Growth Assay of Mutagenized Chlamydomonas reinhardtii Compatible With the International Space Station Veggie Plant Growth Chamber

Frontiers in Plant Science
Junya ZhangA. Mark Settles

Abstract

A biological life support system for spaceflight would capture carbon dioxide waste produced by living and working in space to generate useful organic compounds. Photosynthesis is the primary mechanism to fix carbon into organic molecules. Microalgae are highly efficient at converting light, water, and carbon dioxide into biomass, particularly under limiting, artificial light conditions that are a necessity in space photosynthetic production. Although there is great promise in developing algae for chemical or food production in space, most spaceflight algae growth studies have been conducted on solid agar-media to avoid handling liquids in microgravity. Here we report that breathable plastic tissue culture bags can support robust growth of Chlamydomonas reinhardtii in the Veggie plant growth chamber, which is used on the International Space Station (ISS) to grow terrestrial plants. Live cultures can be stored for at least 1 month in the bags at room temperature. The gene set required for growth in these photobioreactors was tested using a competitive growth assay with mutations induced by short-wave ultraviolet light (UVC) mutagenesis in either wild-type (CC-5082) or cw15 mutant (CC-1883) strains at the start of the assay. Geno...Continue Reading

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Citations

Mar 16, 2021·Critical Reviews in Biotechnology·Matthew J McNultyKaren A McDonald

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Methods Mentioned

BETA
ISS
light microscopy
Assay
genotyping

Software Mentioned

AgriGO
GATK
SNPGenie
Trimmomatic
Picard MarkDuplicates
RealignerTargetCreator
LoFreq
SnpEff
mem
FreeBayes

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