PMID: 2108731Apr 9, 1990Paper

Competitive inhibition by glucose of myo-inositol incorporation into cultured porcine aortic endothelial cells

Biochimica Et Biophysica Acta
B OlgemöllerK D Gerbitz

Abstract

To explore the significance of hyperglycaemia as a causal factor for the appearance of diabetic angiopathies we investigated aspects of myo-inositol metabolism in porcine aortic endothelial cells. myo-Inositol was shown to be a long-living metabolite. Its uptake into the cells was mediated by a high-affinity, Na(+)-dependent uptake system inhibitable by ouabain with an apparent KM of 18.6 mumols/l, which was responsible for more than 80% of total uptake at physiological myo-inositol concentrations. Inhibition of inositol uptake by D-glucose was exclusively competitive with an apparent Ki of 24 mmol/l as shown by Lineweaver-Burk- and Dixon-plot analysis. The specificity of competitive inhibition was studied. L-Glucose which is stereochemically related to myo-inositol in the same way as the D-isomer proved to be an equally potent inhibitor. The hexoses D-galactose, D-mannose and D-fructose inhibited myo-inositol uptake to a minor extent. D-allose and 3-O-methyl-D-glucose had no inhibitory effect indicating that the OH-group of the carbon atom in 3 position is essential for the interaction with the carrier. The acyclic hexitol sorbitol also did not compete. As expected, the aldose reductase blocker sorbinil did not influence the c...Continue Reading

References

Aug 12, 1976·The New England Journal of Medicine·R Ross, J A Glomset
Jan 1, 1989·Metabolism: Clinical and Experimental·M A Yorek, J A Dunlap
Mar 5, 1987·The New England Journal of Medicine·D A GreeneA A Sima
May 1, 1986·Archives of Biochemistry and Biophysics·M A YorekB H Ginsberg
Feb 20, 1986·The New England Journal of Medicine·R Ross
Dec 1, 1985·Molecular and Cellular Endocrinology·B OlgemöllerO H Wieland
Apr 1, 1983·The Journal of Clinical Investigation·G L KingL I Rand
Mar 1, 1984·Progress in Cardiovascular Diseases·N B Ruderman, C Haudenschild
Sep 1, 1984·Analytical Biochemistry·L C MacGregor, F M Matschinsky
Apr 30, 1984·Biochemical and Biophysical Research Communications·S SegalD Pleasure
Apr 1, 1980·The Journal of Clinical Investigation·B A MolitorisW H Daughaday
Aug 1, 1953·The Biochemical Journal·M DIXON

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Citations

Jan 1, 1993·Diabetic Medicine : a Journal of the British Diabetic Association·M A YorekE Davidson
May 15, 1993·Experientia·R Timpl
Nov 11, 2010·Chembiochem : a European Journal of Chemical Biology·Zhuo LuanYoshiyuki Suzuki
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·A Bhatnagar, S K Srivastava
Jun 15, 2013·Biochimie·Marine L Croze, Christophe O Soulage
Jul 1, 1992·Kidney International·N J Guzman, F T Crews
May 6, 1999·Diabetic Medicine : a Journal of the British Diabetic Association·K SuzukiN Hotta
Jun 29, 2004·The Journal of Eukaryotic Microbiology·Michael C Kersting, Phillip E Ryals
Jul 26, 2014·Experimental Biology and Medicine·Hao-Han ChangKerry M Loomes
Jun 30, 1993·Biochimica Et Biophysica Acta·D GaniJ Bramham

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