Competitive Lrp and Dam assembly at the pap regulatory region: implications for mechanisms of epigenetic regulation

Journal of Molecular Biology
Stacey N Peterson, Norbert O Reich

Abstract

Escherichia coli DNA adenine methyltransferase (Dam) and Leucine-responsive regulatory protein (Lrp) are key regulators of the pap operon, which codes for the pilus proteins necessary for uropathogenic E. coli cellular adhesion. The pap operon is regulated by a phase variation mechanism in which the methylation states of two GATC sites in the pap regulatory region and the binding position of Lrp determine whether the pilus genes are expressed. The post-replicative reassembly of Dam, Lrp, and the local regulator PapI onto a hemimethylated pap intermediate is a critical step of the phase variation switching mechanism and is not well understood. We show that Lrp, in the presence and in the absence of PapI and nonspecific DNA, specifically protects pap regulatory GATC sites from Dam methylation when allowed to compete with Dam for assembly on unmethylated and hemimethylated pap DNA. The methylation protection is dependent upon the concentration of Lrp and does not occur with non-regulatory GATC sites. Our data suggest that only at low Lrp concentrations will Dam compete effectively for binding and methylation of the proximal GATC site, leading to a phase switch resulting in the expression of pili.

References

Dec 1, 1976·Journal of Bacteriology·M G Marinus
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B A BraatenD A Low
Mar 1, 1992·Journal of Bacteriology·E BoyeA Løbner-Olesen
Jan 1, 1983·Journal of Bacteriology·S Urieli-ShovalA Razin
May 1, 1994·Molecular Microbiology·L J RasmussenA Løbner-Olesen
Jan 1, 1996·Annual Review of Biochemistry·P Modrich, R Lahue
Dec 1, 1996·Journal of Bacteriology·J R LandgrafJ M Calvo
Nov 13, 1998·Journal of Bacteriology·M van der WoudeD A Low
Mar 27, 2001·EMBO Reports·E BoyeK Skarstad
Aug 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aaron HerndayDavid Low
Oct 1, 2004·The Journal of Biological Chemistry·Neda MashhoonNorbert Reich
Apr 2, 2005·Current Opinion in Microbiology·Anders Løbner-OlesenMartin G Marinus
Dec 3, 2005·Journal of Molecular Biology·Stacey N Peterson, Norbert O Reich
Jan 16, 2007·Journal of Molecular Biology·Stephanie de los Rios, John J Perona
Apr 25, 2007·Infection and Immunity·Nicola HoldenDavid L Gally
May 15, 2007·Journal of Molecular Biology·Stacey N PetersonNorbert O Reich

❮ Previous
Next ❯

Citations

Jun 9, 2009·Nature Reviews. Microbiology·Kirk W DeitschJames R Stringer
May 11, 2012·Nucleic Acids Research·Aswin Sai Narain SeshasayeeSandeep Krishna
Feb 2, 2010·Journal of Bacteriology·Renata Kaminska, Marjan W van der Woude
Feb 5, 2011·Current Opinion in Microbiology·Marjan W van der Woude
Sep 29, 2009·Current Opinion in Microbiology·Justine Collier
Sep 29, 2009·Veterinary Microbiology·Kun SunLi Sun
Jul 15, 2011·Molecular Microbiology·Richard GravelineJosée Harel
May 25, 2011·Journal of Cellular Biochemistry·Wanyan DengJianping Xie
Jul 5, 2011·Molecular Microbiology·Gou FurusawaPatricia Hartzell
Nov 14, 2018·Journal of Bacteriology·Grace M KronerPeter L Freddolino
Jun 8, 2012·Nature Communications·Christina KahramanoglouAswin S N Seshasayee
Apr 18, 2013·The Journal of Biological Chemistry·Josep Casadesús, David A Low
Nov 16, 2019·Nature Reviews. Microbiology·María A Sánchez-Romero, Josep Casadesús
Mar 15, 2018·EcoSal Plus·Glenn T Werneburg, David G Thanassi
Jun 22, 2021·Critical Reviews in Biochemistry and Molecular Biology·Christine A Ziegler, Peter L Freddolino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.