PMID: 1680170May 1, 1991

Competitive protein-binding radioassay of thiamine in simple solutions and in multivitamin pharmaceuticals

The Journal of Pharmacy and Pharmacology
A KozikI Turyna


A competitive inhibition radioassay of thiamine is described using a gel obtained by coupling a buckwheat-seed thiamine-binding protein to CNBr-activated Sepharose. The sample to be analysed is incubated with gel suspension and [14C]thiamine and after centrifugation the radioactivity of the supernatant is measured. The method is simple and specific, and applicable over a thiamine concentration range 0.5-5 microM with a coefficient of variation typically below 5%. The gel is reusable and stable for several months. Applicability of the method for direct determination of thiamine in multivitamin pharmaceuticals is demonstrated.


Oct 1, 1985·Clinical Biochemistry·D S Lee, B W Griffiths
Sep 1, 1982·Analytical Biochemistry·S E LotterH B White

Related Concepts

Thiamine-binding protein
Carrier Proteins
Plasma Protein Binding Capacity
Fluorescence Spectroscopy
Thiamine Mononitrate

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