Competitive translation efficiency at the picornavirus type 1 internal ribosome entry site facilitated by viral cis and trans factors

Journal of Virology
Elena DobrikovaMatthias Gromeier

Abstract

Enteroviruses (EVs) overcome their host cells by usurping the translation machinery to benefit viral gene expression. This is accomplished through alternative translation initiation in a cap-independent manner at the viral internal ribosomal entry site (IRES). We have investigated the role of cis- and trans-acting viral factors in EV IRES translation in living cells. We observed that considerable portions of the viral genome, including the 5'-proximal open reading frame and the 3' untranslated region, contribute to stimulation of IRES-mediated translation. With the IRES in proper context, translation via internal initiation in uninfected cells is as efficient as at capped messages with short, unstructured 5' untranslated regions. IRES function is enhanced in cells infected with the EV coxsackievirus B3, but the related poliovirus has no significant stimulatory activity. This differential is due to the inherent properties of their 2A protease and is not coupled to 2A-mediated proteolytic degradation of the eukaryotic initiation factor 4G. Our results suggest that the efficiency of alternative translation initiation at EV IRESs depends on a properly configured template rather than on targeted alterations of the host cell translat...Continue Reading

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A NomotoE Wimmer
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J Hambidge, P Sarnow
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E E WyckoffE Ehrenfeld
Nov 1, 1985·Molecular and Cellular Biology·H D BernsteinD Baltimore
Dec 1, 1995·Genes & Development·S Z Tarun, A B Sachs
Jan 1, 1996·Molecular and Cellular Biology·R L Tanguay, D R Gallie
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·M GromeierE Wimmer
May 1, 1997·BioTechniques·A Y Borovkov, M I Rivkin
Jan 7, 1998·Molecular and Cellular Biology·A GradiN Sonenberg
Aug 14, 1998·Molecular Cell·S E WellsA B Sachs
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A GradiN Sonenberg
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·C J WiluszS W Peltz
Feb 1, 2002·The Journal of Biological Chemistry·Christopher A BradleyRobert E Rhoads
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena DobrikovaMatthias Gromeier
Oct 28, 2004·Cell·Geoff A Otto, Joseph D Puglisi
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Patrick HundsdoerferMatthias W Hentze

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Citations

Jul 22, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christian GoetzMatthias Gromeier
Nov 3, 2006·Journal of Virology·Stephanie A CampbellMatthias Gromeier
Apr 1, 2011·Journal of Virology·Eric S WangMatthias Gromeier
Oct 11, 2011·Future Virology·Christian GoetzMatthias Gromeier
May 15, 2009·Biochimica Et Biophysica Acta·Michael Niepmann
Apr 19, 2008·Trends in Microbiology·Encarnación Martínez-Salas
Jan 15, 2010·The Journal of General Virology·Bhupendra VermaSaumitra Das
Aug 5, 2008·Virus Research·Graham J Belsham
Feb 15, 2008·The Journal of General Virology·Encarnación Martínez-SalasNoemi Fernandez

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