Complement-activating antibodies in sera from infected individuals and vaccinated volunteers that target human immunodeficiency virus type 1 to complement receptor type 1 (CR1, CD35)

Virology
J Zhou, David C Montefiori

Abstract

Complement receptor type 1 (CR1) plays a central role in clearing immune complexes from the circulation and probably contributes to the retention of immune complexes on the surface of follicular dendritic cells. Virus-specific, complement-activating antibodies can target human immunodeficiency virus type 1 (HIV-1) to CR1-bearing cells but the potential impact that these antibodies have on HIV-1 pathogenesis is unknown. To study these antibodies, an assay was developed in which immune complexes containing HIV-1, antibody, and complement were formed in vitro and captured on the surface of 96-well immunoplates coated with recombinant soluble human CR1 (rsCR1). Captured virus was detected by p24 immunoassay or by infection of human CD4+ lymphocytes. Two laboratory strains of HIV-1 (IIIB and MN) and primary isolates could be captured using sera from infected individuals or vaccinated volunteers as a source of complement-activating antibodies. HIV-1 immune complexes captured by solid-phase rsCR1 could be transferred to MT-2 cells for productive infection. Antibodies had no activity in this assay when the normal human serum used as a source of complement had been heat-inactivated or depleted of complement component C3, confirming a re...Continue Reading

Citations

May 1, 2007·Current Opinion in HIV and AIDS·David C MontefioriJohn R Mascola
Jul 10, 2002·Lancet·Christoph HessJürg-Alfred Schifferli
Mar 11, 2015·PLoS Neglected Tropical Diseases·Sarah A C FalcãoCamila I de Oliveira
Feb 7, 2013·Medical Hypotheses·Mojtaba FarjamBahareh Fakhraei
Jun 30, 2007·Journal of Internal Medicine·M Huber, A Trkola
Jan 6, 2010·Current Opinion in HIV and AIDS·Heribert Stoiber

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