Complement Activation by Pulp Capping Materials Plays a Significant Role in Both Inflammatory and Pulp Stem Cells' Recruitment

Journal of Endodontics
Thomas GiraudI About

Abstract

The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment. Conditioned media were prepared from 3 pulp capping biomaterials: Biodentine (Septodont, Saint-Maur-des-Fosses, France), TheraCal (BISCO, Lançon De Provence, France), and Xeno III (Dentsply Sirona, Versaille, France). Injured pulp fibroblasts were cultured with these conditioned media to analyze C5a secretion using an enzyme-linked immunosorbent assay. Dental pulp stem cells (DPSCs) were isolated from human third molar explants by magnetic cell sorting with STRO-1 antibodies. The expression of C5a receptor on DPSCs and inflammatory (THP-1) cells was investigated by immunofluorescence. The migration of both DPSCs and THP-1 cells was studied in Boyden chambers. Pulp fibroblast production of C5a significantly increased when the cells were incubated with ...Continue Reading

Citations

Oct 28, 2019·Clinical Oral Investigations·Charlotte JeanneauImad About
Sep 21, 2020·Journal of Endodontics·Chloé Le FournisImad About
Sep 21, 2020·Journal of Endodontics·Nastaran MeschiNikita B Ruparel
Oct 15, 2020·Clinical Oral Investigations·Madison BergmannImad About
Apr 8, 2019·Journal of Endodontics·Chloé Le FournisImad About
Oct 17, 2017·Journal of Endodontics·Charlotte JeanneauImad About
Mar 12, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Mariano S PedanoBart Van Meerbeek
Oct 3, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Thomas GiraudImad About
Oct 15, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Eirini AthanasiadouAthina Bakopoulou
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mariano S PedanoBart Van Meerbeek
Jun 29, 2021·Journal of Endodontics·Chloé Le FournisImad About

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