Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.

PloS One
Zhiping WangYan An

Abstract

Most fish embryos that develop externally are exposed to an environment full of microbes. How they survive microbial attacks are not understood to date. Here we demonstrated that the egg cytosol prepared from the newly fertilized eggs of zebrafish Danio rerio is capable of killing the Gram-negative bacterium Escherichia coli, via in vitro assay system of the complement activity established. All findings indicate that it is the complement system operating via the alternative pathway that is attributable to the bacteriolytic activity. This is the first report providing the evidence for the functional role of the maternal complement components in fish eggs, paving the way for study of maternal immunity in other organisms whose eggs are fertilized in vitro.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 27, 1985·Journal of Immunological Methods·H Van DijkJ M Willers
Jan 1, 1995·Critical Reviews in Clinical Laboratory Sciences·B P Morgan
Sep 1, 1993·Developmental and Comparative Immunology·A CastilloA J Villena
Jun 25, 1998·Developmental and Comparative Immunology·T L KoppenhefferH M Falvo
Sep 22, 1998·Current Biology : CB·M Robertson
Jul 25, 2000·Immunopharmacology·U Kishore, K B Reid
Oct 17, 2001·Developmental and Comparative Immunology·I K ZarkadisJ D Lambris
Jun 24, 2004·Fish & Shellfish Immunology·Miguel Rubio-GodoyRichard C Tinsley
Oct 7, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bergljót MagnadóttirSigridur Gudmundsdóttir
Feb 3, 2005·Fish & Shellfish Immunology·Terje EllingsenRoy A Dalmo
Jun 9, 2005·Fish & Shellfish Immunology·A ZapataA Cortés
Jun 14, 2005·Fish & Shellfish Immunology·H BoshraJ O Sunyer
Sep 20, 2005·Fish & Shellfish Immunology·Heidi B T HuttenhuisJan H W M Rombout
Apr 24, 2007·Fish & Shellfish Immunology·Marie LøvollRoy A Dalmo

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Citations

Sep 22, 2010·Marine Biotechnology·Jun Zhang, Shicui Zhang
Oct 17, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Chubin QinZhigang Zhou
Jul 30, 2011·Diseases of Aquatic Organisms·L Al-Hussinee, J S Lumsden
Jun 13, 2020·Frontiers in Immunology·Prabhugouda SiriyappagouderJorge M O Fernandes
Jul 30, 2010·Functional & Integrative Genomics·Guanni SunShicui Zhang
Jul 16, 2014·Zebrafish·Amorce LimaBurt Anderson
Dec 4, 2012·International Journal of Molecular Sciences·Lei GaoWeidong Liu
Sep 5, 2013·Infection and Immunity·Tomasz K PrajsnarStéphane Mesnage
Jun 13, 2014·Integrative Zoology·Chunxin FanJiakun Song
Jun 30, 2019·Developmental and Comparative Immunology·Wenche JohansenKnut Olav Strætkvern
Mar 19, 2011·Developmental and Comparative Immunology·Miki NakaoTomonori Somamoto
Jul 16, 2014·BMC Genomics·Adrijana ŠkugorØivind Andersen
May 16, 2009·Fish & Shellfish Immunology·P Swain, S K Nayak

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Methods Mentioned

BETA
electrophoresis

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