Complement C1q Interacts With LRP1 Clusters II and IV Through a Site Close but Different From the Binding Site of Its C1r and C1s-Associated Proteases.

Frontiers in Immunology
Guillaume FouëtVéronique Rossi

Abstract

LRP1 is a large endocytic modular receptor that plays a crucial role in the scavenging of apoptotic material through binding to pattern-recognition molecules. It is a membrane anchored receptor of the LDL receptor family with 4 extracellular clusters of ligand binding modules called cysteine rich complement-type repeats that are involved in the interaction of LRP1 with its numerous ligands. Complement C1q was shown to interact with LRP1 and to be implicated in the phagocytosis of apoptotic cells. The present work aimed at exploring how these two large molecules interact at the molecular level using a dissection strategy. For that purpose, recombinant LRP1 clusters II, III and IV were produced in mammalian HEK293F cells and their binding properties were investigated. Clusters II and IV were found to interact specifically and efficiently with C1q with K Ds in the nanomolar range. The use of truncated C1q fragments and recombinant mutated C1q allowed to localize more precisely the binding site for LRP1 on the collagen-like regions of C1q (CLRs), nearby the site that is implicated in the interaction with the cognate protease tetramer C1r2s2. This site could be a common anchorage for other ligands of C1q CLRs such as sulfated proteo...Continue Reading

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Citations

Jun 3, 2021·International Journal of Molecular Sciences·Guillaume FouëtVéronique Rossi
Jul 31, 2021·FEBS Open Bio·Catherine Wicker-PlanquartNicole M Thielens

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Methods Mentioned

BETA
RAP
dissection
PCR
gel filtration
surface
chips
chip
flow cytometry
scraping
surface plasmon resonance

Software Mentioned

BIAevaluation
IQ
Expasy
PROTPARAM

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