PMID: 11341920May 9, 2001Paper

Complement C4bC2 complex formation: an investigation by surface plasmon resonance

Biochimica Et Biophysica Acta
A Laich, R B Sim

Abstract

Complex formation between the human complement proteins C4b and C2 was investigated by surface plasmon resonance. C4b was immobilised and C2 was used in the fluid phase to measure interaction at different ionic strengths (30-830 mM NaCl) and in the absence and presence of MgCl2. Maximum binding was observed at 30 mM NaCl, and was negligible above 300 mM NaCl. Binding was not greatly influenced by variation in Mg(2+) in the range of 2.5-15 mM. C4bC2 affinity (Kd) was determined by steady-state analysis to be 7.2x10(-8) M in physiological conditions (10 mM Hepes, 2.5 mM MgCl2, 0.75 mM CaCl2 and 140 mM NaCl, pH 7.4). For C4(H2O)C2 complex formation, a Kd of 4.0x10(-8) M was calculated. As far as detected by the applied method, complex formation does not involve conformational changes of one of the binding partners. Consistent with previous reports, C4bC2 binding takes place as a multiple-site binding event in the presence of Mg2+. C4bC2 complex formation in 10 mM Hepes, 2.5 mM EDTA and 140 mM NaCl (pH 7.4) was also observed and the interaction showed characteristics of a single-site binding event. Kd was 1.5x10(-8) M. Complement factor B (FB) was also tested for its binding to immobilised C4b. Weak interaction was observed at FB c...Continue Reading

References

Jan 1, 1986·Molecular Immunology·E L Pryzdial, D E Isenman
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A C ChanJ P Atkinson
Aug 25, 1995·The Journal of Biological Chemistry·D E HourcadeT J Oglesby
Jan 1, 1993·Methods in Enzymology·A W Dodds
Jan 4, 1993·Journal of Immunological Methods·S C Williams, R B Sim

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Citations

Dec 21, 2002·Molecular Immunology·Anja RoosMohamed R Daha
Feb 25, 2009·Acta Crystallographica. Section D, Biological Crystallography·Vengadesan KrishnanSthanam V L Narayana
Aug 9, 2008·Protein Science : a Publication of the Protein Society·Nurit HaspelJohn D Lambris
Apr 17, 2009·Structure·Vengadesan KrishnanSthanam V L Narayana
Apr 8, 2006·Biochemical and Biophysical Research Communications·Kwok-Min HuiJameel M Inal
Dec 26, 2002·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Dec 26, 2016·Biochimica Et Biophysica Acta. General Subjects·Johara Boldrini-FrançaEliane Candiani Arantes
Aug 9, 2005·Nature Immunology·Suzan H M RooijakkersJos A G van Strijp
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Géza AmbrusPéter Závodszky
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hani BoshraJ Oriol Sunyer
Aug 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alessandra ZarantonelloGregers R Andersen
Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jameel M InalJürg-A Schifferli
Aug 9, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Cecilia VahlbergKajsa Uvdal
Jan 31, 2004·Analytical Chemistry·Jing-Xue ShiCheng-Gang Zhang

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