Complement C9 binding site and the anti-microbial activity of caprine vitronectin are localized in close proximity in the N-terminal region of the protein.

Microbial Pathogenesis
Prasanta Kumar Koustasa MishraParitosh Joshi

Abstract

Vitronectin (Vn) is a ligand for complement C9 and modulates its activity that favors bacterial growth and survival. At the same time, the anti-microbial activity of the heparin-binding region of human Vn has been documented. To understand these diverse and opposite functions of the protein, we have analyzed the interaction of caprine Vn with C9 in the homologous system. In a previous study, the C9 binding activity was mapped to the N-fragment of the caprine Vn (N-Vn), representing the first 200 amino acids. Interestingly, this fragment also inhibited bacterial growth. In this study, we have generated four sub-fragments of N-Vn and analyzed C9 binding by ELISA, blot overlay, surface plasmon resonance and circular dichroism spectroscopy. These sub-fragments were also tested for antimicrobial activity against E. coli and S. aureus by drop plate method and analyzing cell death by flow cytometry. Results of these analyses together with previous data suggest that in addition to the second RGD motif (106-108 amino acids), the first 47 residues are also required for C9 binding. The anti-microbial tests employed indicate that the growth inhibitory property is contributed by 101-150 residues of Vn. These results provide an initial insig...Continue Reading

References

Jan 1, 1991·Annual Review of Cell Biology·K T Preissner
Jul 1, 1995·Clinical and Experimental Immunology·M SheehanJ A Charlesworth
Apr 1, 1993·Clinical and Experimental Immunology·L MilisB A Pussell
May 23, 1997·The Journal of Biological Chemistry·D Seiffert, J W Smith
Jul 10, 1999·The International Journal of Biochemistry & Cell Biology·I SchvartzS Shaltiel
Mar 11, 2004·European Journal of Biochemistry·Emma AnderssonArtur Schmidtchen
Sep 30, 2004·Nature Immunology·Michael C Carroll
May 30, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Martin MalmstenArtur Schmidtchen
Jun 15, 2007·Protein Science : a Publication of the Protein Society·Aiwu Zhou
Jul 25, 2007·BMC Bioinformatics·Sneh LataG P S Raghava
Sep 4, 2007·Protein Science : a Publication of the Protein Society·Magnus KjaergaardMichael Ploug
Dec 7, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Manish Mahawar, Paritosh Joshi
Sep 5, 2009·Nature Reviews. Immunology·Peter F Zipfel, Christine Skerka
Nov 1, 1938·The Journal of Hygiene·A A MilesJ O Irwin
Sep 3, 2010·Molecular Microbiology·Birendra SinghKristian Riesbeck

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