PMID: 15383587Sep 24, 2004Paper

Complement components C5 and C7: recombinant factor I modules of C7 bind to the C345C domain of C5

The Journal of Immunology : Official Journal of the American Association of Immunologists
Chuong-Thu Thai, R T Ogata

Abstract

Studies reported over 30 years ago revealed that latent, nonactivated C5 binds specifically and reversibly to C6 and C7. These reversible reactions are distinct from the essentially nonreversible associations with activated C5b that occur during assembly of the membrane attack complex, but they likely involve some, perhaps many, of the same molecular contacts. We recently reported that these reversible reactions are mediated by the C345C (NTR) domain at the C terminus of the C5 alpha-chain. Earlier work by others localized the complementary binding sites to a tryptic fragment of C6 composed entirely of two adjacent factor I modules (FIMs), and to a larger fragment of C7 composed of its homologous FIMs as well as two adjoining short consensus repeat modules. In this work, we expressed the tandem FIMs from C7 in bacteria. The mobility on SDS-polyacrylamide gels, lack of free sulfhydryl groups, and atypical circular dichroism spectrum of the recombinant product rC7-FIMs were all consistent with a native structure. Using surface plasmon resonance, we found that rC7-FIMs binds specifically to both C5 and the rC5-C345C domain with K(D) approximately 50 nM, and competes with C7 for binding to C5, as expected for an active domain. Thes...Continue Reading

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Citations

Dec 16, 2004·The Journal of Biological Chemistry·Janice BramhamPaul N Barlow
Apr 14, 2012·The Journal of Biological Chemistry·Alexander E AleshinRobert C Liddington
Feb 5, 2016·Nature Communications·Marina SernaDoryen Bubeck
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chuong-Thu Thai, Ronald T Ogata
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jul 29, 2009·Biomolecular NMR Assignments·Marie M PhelanJanice Bramham
Aug 10, 2006·Immunogenetics·Masaru Nonaka, Ayuko Kimura
Apr 5, 2007·European Journal of Immunology·Marie-Anne Rameix-WeltiVeronique Frémeaux-Bacchi
Apr 2, 2011·The Journal of Biological Chemistry·Leslie L LovelaceLukasz Lebioda
Jun 10, 2008·Nature Immunology·Folmer FredslundGregers R Andersen
Mar 16, 2021·Molecular Genetics and Genomics : MGG·Lisen LiJiale Li
Nov 8, 2005·Molecular Immunology·Maria P ChondrouIoannis K Zarkadis
Apr 21, 2007·Journal of Molecular Biology·Pietro RoversiSusan M Lea
Jan 14, 2010·Developmental and Comparative Immunology·Angeliki Mikrou, Ioannis K Zarkadis

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