Complement Expression and Activation in Osteoarthritis Joint Compartments.

Frontiers in Immunology
Elisa AssirelliRiccardo Meliconi

Abstract

To investigate complement(C) factors(F) and their activation fragments expression in OA joint tissues. Immunohistochemistry and quantitative imaging were performed to analyze C3, C4, and CF (factor) B expression on osteochondral biopsies (43 patients) collected during arthroplasty. Isolated chondrocytes and synoviocytes, cartilage and synovial tissues obtained from surgical specimens of OA patients (15 patients) were cultured with or without IL-1β. Real time PCR for CFB, C3, and C4 was performed. Culture supernatants were analyzed for C3a, C5a, CFBa, and terminal complement complex (TCC) production. In osteochondral biopsies, C factor expression was located in bone marrow, in a few subchondral bone cells and chondrocytes. C3 was the most expressed while factor C4 was the least expressed factor. Gene expression showed that all C factors analyzed were expressed both in chondrocytes and synoviocytes. In chondrocyte cultures and cartilage explants, CFB expression was significantly higher than C3 and C4. Furthermore, CFB, but not C3 and C4 expression was significantly induced by IL-1β. As to C activation factors, C3a was the most produced and CFBa was induced by IL-1β in synovial tissue. TCC production was undetectable in isolated c...Continue Reading

References

Jul 1, 1988·Annals of Internal Medicine·D T FelsonR F Meenan
Jan 1, 1997·Clinical and Experimental Immunology·B P Morgan, P Gasque
Mar 17, 2005·Rheumatology International·Tania SilvestriRiccardo Meliconi
Dec 25, 2009·Pain·Jun Ho JangTimothy J Brennan
Jan 29, 2010·Clinical Rheumatology·Ana Luisa G CalichRicardo Fuller
Sep 15, 2010·Cell and Tissue Research·J Vidya Sarma, Peter A Ward
Oct 7, 2010·Nature Reviews. Rheumatology·Jérémie Sellam, Francis Berenbaum
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Nov 8, 2011·Nature Medicine·Qian WangWilliam H Robinson
Jan 24, 2012·Best Practice & Research. Clinical Rheumatology·Farshid Guilak
May 4, 2012·Drugs & Aging·Zdravko JotanovicZlatko Dembic
Jan 31, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Abdul Haseeb, Tariq M Haqqi
Feb 14, 2013·Advances in Experimental Medicine and Biology·Mariusz Z RatajczakAnna Janowska-Wieczorek
May 4, 2013·Therapeutic Advances in Musculoskeletal Disease·Jeremy Sokolove, Christin M Lepus
Jun 19, 2013·Seminars in Immunology·Markus Huber-LangAnita Ignatius
Jun 1, 2012·European Journal of Microbiology & Immunology·P N NesargikarR Chavez
Jun 20, 2014·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Elisa AssirelliLia Pulsatelli
May 17, 2015·Trends in Immunology·Mihály JózsiSantiago Rodríguez de Córdoba
Oct 6, 2015·World Journal of Stem Cells·Ingrid U SchraufstatterRichard G DiScipio
Dec 5, 2015·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Qiang JiaoXiaochun Wei
Feb 6, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Stefan KluzekNigel K Arden
Feb 9, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Andreza U Quadros, Thiago M Cunha
Aug 20, 2016·Nature Reviews. Rheumatology·William H RobinsonJeremy Sokolove
Mar 2, 2017·Clinical and Experimental Immunology·R LubbersL A Trouw
Feb 13, 2018·Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders·Sandeep SilawalGundula Schulze-Tanzil
Sep 16, 2018·Molecular Immunology·Chrysostomi GialeliAnna M Blom
Jan 22, 2020·Arthritis & Rheumatology·Christopher M DunnMatlock A Jeffries

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
biopsy
PCR

Software Mentioned

SPSSv
NIS
Elements
GraphPad prism
primer
- Blast

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.