PMID: 3745932Jan 1, 1986Paper

Complement fixation by pemphigus antibody. III. Altered epidermal cell membrane integrity mediated by pemphigus antibody and complement

The Journal of Investigative Dermatology
S KawanaR E Jordon

Abstract

The present study investigates the effects of pemphigus IgG and complement upon cell viability and/or membrane integrity using trypan blue exclusion, ethidium bromide (EB) staining, and fluorescein diacetate (FDA) conversion by living cells. Forty-eight-hour cultivated epidermal monolayers of neonatal BALB/c mice were incubated in media containing 1 mg/ml purified pemphigus IgG for 48 h in either the presence or absence of complement (absorbed AB sera). Adherent and detached cells were examined by both phase and fluorescence microscopy. Results from trypan blue exclusion showed that pemphigus IgG plus complement produced a modest decrease in exclusion of the dye compared to pemphigus IgG without complement. When FDA/EB comparisons were made, however, the differences were more substantial. When complement plus pemphigus IgG was added to cultures, the number of FDA-positive adherent cells decreased significantly and the number of EB-positive detached cells increased significantly. The effects of complement were inhibited by the use of heat-inactivated AB sera or by C1q depletion of AB sera. No significant effect on the cells was observed in the presence or absence of complement when pemphigus F(ab')2 fragments or when normal IgG ...Continue Reading

Citations

Jan 1, 1990·Archives of Dermatological Research·S KawanaS Nishiyama
Mar 20, 2004·Veterinary Dermatology·Wayne S Rosenkrantz
Apr 25, 2018·Frontiers in Immunology·Valéria Bumiller-BiniAngelica Beate Winter Boldt
Dec 1, 1989·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·D M Williams
Feb 1, 1987·Journal of the American Academy of Dermatology·P D CruzR D Sontheimer

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