Complement-independent adherence of Escherichia coli to complement receptors in vitro

The Journal of Clinical Investigation
D P FineJ C Guckian

Abstract

We studied adherence to human cells by a strain of Escherichia coli. Adherence to erythrocytes was assessed directly by phase-contrast microscopy and indirectly by hemagglutination; adherence to peripheral blood leukocytes, using radiolabeled bacteria and subsequent determination of leukocyte-associated radioactivity; and adherence to renal glomeruli, by microscopy of fluoresceinated bacteria and of Gram-stained nonfluoresceinated bacteria. In serum-free systems, E. coli of this strain adhered to human erythrocytes, which have surface receptors for the third component of complement (C3), but not to erythrocytes from species lacking this receptor. 1 mM trypan blue, a reagent that inhibits complement receptor function, inhibited adherence to human erythrocytes, as well as adherence to leukocytes and glomeruli. Preincubation of erythrocytes and leukocytes with complement-coated zymosan particles partially blocked subsequent bacterial adherence. Incubation of human erythrocytes with aging human serum, with trypsin-cleaved C3, or with C3 cleaved by the classical pathway convertase (EAC142)-all of which treatments deposited C3 on the erythrocyte surface, presumably at C3 receptors-inhibited subsequent E. coli adherence. Finally, incu...Continue Reading

References

Sep 1, 1978·The American Journal of Medicine·B A Waisbren
Nov 1, 1977·The Journal of Experimental Medicine·I E Salit, E C Gotschlich
Feb 1, 1977·The Journal of Experimental Medicine·A G Ehlenberger, V Nussenzweig
Oct 1, 1975·The Journal of Experimental Medicine·M C GelfandI Green
Jul 1, 1976·The New England Journal of Medicine·M C GelfandM M Frank

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Citations

Sep 12, 2018·Bioscience Reports·Alfonso Olivos-GarcíaRuy Pérez-Tamayo
Aug 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·V JønssonA Videbaek

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