Abstract
Vitronectin (complement S-protein), a multifunctional glycoprotein, inhibits complement-mediated cytolysis at two identified stages of terminal complement complex (TCC) formation: blocking of C5b-7 membrane binding, and prevention of C9 polymerization. However, the functional domain(s) of vitronectin involved in these reactions remains incompletely defined. In order to identify the complement inhibition site, a 12-kD heparin binding fragment and two other internal fragments (53 kD and 43 kD) of vitronectin were isolated after cyanogen bromide (CNBr) treatment of the native molecule. Potent inhibition of guinea pig erythrocyte (GPE) reactive lysis was demonstrated with native vitronectin, total CNBr digest and the 53-kD and 43-kD fragments, but only very poorly by the heparin binding 12-kD peptide. Similarly, the 43-kD fragment blocked the binding of C5b-7 to immobilized vitronectin, whereas the 12-kD fragment had no effect. These data localize the C5b-7 binding site to a 43-kD internal region. Further characterization of the fragments was carried out in an assay which detected C9 polymerization in the presence of C5b-8. Polymerized material was separated by PAGE, detected by autoradiography and quantified after excision from th...Continue Reading
References
Jan 1, 1991·Annual Review of Cell Biology·K T Preissner
Sep 1, 1991·Immunology Today·A ErdeiJ Gergely
Oct 20, 1987·Biochemistry·D Jenne, K K Stanley
May 31, 1988·Biochemistry·J TschoppK T Preissner
Jun 1, 1988·Cell Structure and Function·M IzumiM Hayashi
Aug 1, 1988·Cell Structure and Function·T YatohgoM Hayashi
Mar 15, 1989·Biochemical and Biophysical Research Communications·A Hildebrand
Nov 1, 1989·Blut·K T Preissner
Nov 6, 1989·European Journal of Biochemistry·D JenneW B Huttner
Jan 1, 1989·Complement and Inflammation·A P DalmassoL Raij
Feb 1, 1989·Annals of the Rheumatic Diseases·J A CharlesworthD Wakefield
Sep 1, 1987·Molecular Immunology·J Tschopp, D Masson
Jun 1, 1969·The Biochemical Journal·J J Marchalonis
Apr 1, 1993·Clinical and Experimental Immunology·L MilisB A Pussell
Citations
Oct 7, 2004·Rapid Communications in Mass Spectrometry : RCM·John M KoomenRyuji Kobayashi
May 24, 2005·Infection and Immunity·Malikah AbdullahChristopher Elkins
Sep 3, 2010·Molecular Microbiology·Birendra SinghKristian Riesbeck
Sep 14, 2013·IUBMB Life·David I LeavesleyZee Upton
Mar 15, 2014·PloS One·Crisanto M LopezPeter F Zipfel
Jan 26, 2016·PloS One·Teresia HallströmKristian Riesbeck
Nov 8, 2016·Frontiers in Immunology·Jennifer A Caine, Jenifer Coburn
Nov 1, 2018·Journal of the American Society of Nephrology : JASN·Fengxiao BuRichard J H Smith
Aug 1, 2020·Frontiers in Immunology·Jack MellorsMiles Carroll
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Stefan P W de VriesPeter W M Hermans
Sep 4, 2014·Thrombosis and Haemostasis·Sylvia KohlerSven Hammerschmidt
Jul 6, 2017·Proteomics. Clinical Applications·Nikita GahoiSanjeeva Srivastava
Aug 1, 2018·Frontiers in Microbiology·Magnus PaulssonKristian Riesbeck
Mar 14, 1997·The Journal of Biological Chemistry·P ZhuangC B Peterson
Feb 8, 2013·Molecular Microbiology·Yu-Ching SuKristian Riesbeck
Dec 19, 2017·Cell and Tissue Research·Gurpreet Kaur Aulakh
Jul 28, 2018·Scientific Reports·Kalvis BrangulisKaspars Tars
Jan 22, 2019·Hematology·Jirawan PanachanRuchaneekorn W Kalpravidh
Mar 15, 2020·FEBS Letters·Eric S Krukonis, Joshua J Thomson
Feb 6, 2017·Microbiology and Immunology·Rao T PrasadaJoshi Paritosh
Sep 15, 2015·PloS One·Teresia HallströmPeter F Zipfel
Nov 17, 2020·Frontiers in Immunology·Zahra MalekshahiHeribert Stoiber
Mar 7, 2020·Microbial Pathogenesis·Prasanta Kumar Koustasa MishraParitosh Joshi
Aug 27, 1998·Neuroscience Letters·D G Walker, P L McGeer
Aug 12, 2016·Journal of Virology·Jonas Nascimento CondeRonaldo Mohana-Borges
Jul 7, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·George BoguslawskiCarlos A Labarrere
Oct 8, 2015·Biomacromolecules·Saziye YorulmazNam-Joon Cho