PMID: 7542572Jul 1, 1995Paper

Complement inhibition by human vitronectin involves non-heparin binding domains

Clinical and Experimental Immunology
M SheehanJ A Charlesworth

Abstract

Vitronectin (complement S-protein), a multifunctional glycoprotein, inhibits complement-mediated cytolysis at two identified stages of terminal complement complex (TCC) formation: blocking of C5b-7 membrane binding, and prevention of C9 polymerization. However, the functional domain(s) of vitronectin involved in these reactions remains incompletely defined. In order to identify the complement inhibition site, a 12-kD heparin binding fragment and two other internal fragments (53 kD and 43 kD) of vitronectin were isolated after cyanogen bromide (CNBr) treatment of the native molecule. Potent inhibition of guinea pig erythrocyte (GPE) reactive lysis was demonstrated with native vitronectin, total CNBr digest and the 53-kD and 43-kD fragments, but only very poorly by the heparin binding 12-kD peptide. Similarly, the 43-kD fragment blocked the binding of C5b-7 to immobilized vitronectin, whereas the 12-kD fragment had no effect. These data localize the C5b-7 binding site to a 43-kD internal region. Further characterization of the fragments was carried out in an assay which detected C9 polymerization in the presence of C5b-8. Polymerized material was separated by PAGE, detected by autoradiography and quantified after excision from th...Continue Reading

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Citations

Oct 7, 2004·Rapid Communications in Mass Spectrometry : RCM·John M KoomenRyuji Kobayashi
Sep 3, 2010·Molecular Microbiology·Birendra SinghKristian Riesbeck
Sep 14, 2013·IUBMB Life·David I LeavesleyZee Upton
Nov 1, 2018·Journal of the American Society of Nephrology : JASN·Fengxiao BuRichard J H Smith
Aug 1, 2020·Frontiers in Immunology·Jack MellorsMiles Carroll
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Stefan P W de VriesPeter W M Hermans
Jul 6, 2017·Proteomics. Clinical Applications·Nikita GahoiSanjeeva Srivastava
Dec 19, 2017·Cell and Tissue Research·Gurpreet Kaur Aulakh
Mar 15, 2020·FEBS Letters·Eric S Krukonis, Joshua J Thomson
Nov 17, 2020·Frontiers in Immunology·Zahra MalekshahiHeribert Stoiber
Aug 27, 1998·Neuroscience Letters·D G Walker, P L McGeer
Jul 7, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·George BoguslawskiCarlos A Labarrere

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