PMID: 9435030Jan 1, 1997Paper

Complement-mediated enhancement of HIV-1 infection in peripheral blood mononuclear cells

Scandinavian Journal of Infectious Diseases
S D NielsenJ E Hansen

Abstract

We investigated if complement-mediated enhancement of HIV infection occurs in peripheral blood mononuclear cells (PBMC). In 7 experiments, we evaluated the effect of human complement on HIVIIIB infection in vitro. We measured HIV antigen production on day 4 and found that pre-incubation of HIV with complement led to enhanced production of antigen with a median enhancement of 2.5-fold (range 1.1-6.8). This complement-mediated increase in antigen production was statistically significant (p < 0.02). Complement-mediated enhancement of HIV infection was also tested in CD4 cells enriched from PBMC, and CD4 cells persistently gave higher levels of infection enhancement than PBMC. Thus, CD4 cells appear to be sufficient for complement-mediated enhancement of HIV infection to occur. In addition, we tested if it was possible to detect complement-mediated enhancement of primary HIV isolates in PBMC. We tested 3 isolates and found only a minor effect on antigen production (median enhancement 1.2-fold, range 0.6-1.5). Furthermore, addition of HIV-specific antibodies in combination with complement resulted in enhanced antigen production in 2/3 sera tested. However, the combination of complement and antibodies resulted in only a minor increas...Continue Reading

References

Jan 1, 1990·Cytometry·S MiltenyiA Radbruch
Mar 1, 1993·Immunopharmacology·N ThieblemontN Haeffner-Cavaillon

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Citations

Mar 17, 2010·Cellular & Molecular Immunology·Qigui YuXuebin Qin
Jun 15, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·V BallegaardL Munthe-Fog
Sep 19, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P K Datta, J Rappaport
Apr 24, 2009·Vaccine·Panisadee AvirutnanMichael S Diamond
Dec 2, 2015·PLoS Pathogens·Balthasar A HeestersMichael C Carroll
Jun 21, 2014·Nature Reviews. Immunology·Balthasar A HeestersMichael C Carroll
Aug 1, 2020·Frontiers in Immunology·Jack MellorsMiles Carroll

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