PMID: 46261Mar 1, 1975

Complement-mediated release of histamine from human leukocytes

The Journal of Immunology : Official Journal of the American Association of Immunologists
J A GrantA L Jackson


Activation of either the alternative or classical pathway of complement generated a factor which induced release of histamine from both non-allergic and allergic human basophils. This factor probably is derived from the complement system since 1) its formation was associated with loss of C3 activity in human serum, 2) chemotactic factor, probably also a complement product, was generated simultaneously, 3) heat inactivation blocked its formation, 4) anti-C3 and anti-C5 blocked formation of the factor, and finally 5) anti-C5 inhibited the activity of the factor once it had been formed. It appears that both complement-mediated and allergen-mediated release of histamine from basophils are secretory, non-cytolytic pathways since both were maximal at 37 degrees C, required the presence of divalent cations, and were inhibited by theophylline. One consistent difference between these two mechanisms was noted: complement-initiated release of histamine occurred more quickly.

Related Concepts

Immune complex
Cations, Divalent
Hemolytic Complement
Histamine Release
Immune Sera
Differential White Blood Cell Count Procedure

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