Complement receptor expression on neutrophils at an inflammatory site, the Pseudomonas-infected lung in cystic fibrosis

The Journal of Clinical Investigation
M BergerG Döring

Abstract

Activation of human neutrophils (PMN) is accompanied by rapid upregulation of CR1, the C3b receptor, and CR3, the iC3b receptor, which also serves as the PMN's major adherence protein. This is necessary for migration and phagocytosis, but the extent of expression of these proteins on PMN at inflammatory sites has not been determined. We used monoclonal antibodies and flow cytometry to assess CR1 and CR3 expression on PMN in bronchoalveolar lavage (BAL) fluid of cystic fibrosis (CF) patients chronically infected with pseudomonas and in sterile joint fluid of arthritis patients. Resting peripheral blood PMN from these patients and normals expressed similar low levels of CR1 and CR3, and the patients' PMN increased CR1 and CR3 expression normally when stimulated in vitro. CR3 expression on CF BAL PMN was 90 +/- 12% of that on the same patient's blood cells stimulated in vitro with FMLP. In contrast, CR1 expression on BAL PMN was only 27 +/- 8% of that on stimulated blood cells. Similar results were obtained for joint PMN. This pattern could be reproduced in vitro by treating FMLP-stimulated blood cells with BAL supernatants or with pseudomonas or PMN elastase. The serine protease inhibitors, PMSF and alpha 1-antitrypsin prevented ...Continue Reading

References

Dec 1, 1979·The Journal of Infectious Diseases·S A MurpheyA D Schreiber
Feb 1, 1977·The Journal of Experimental Medicine·A G Ehlenberger, V Nussenzweig
Apr 10, 1986·The New England Journal of Medicine·F D MooreD T Fearon
Feb 21, 1985·The New England Journal of Medicine·M A ArnaoutH R Colten
Nov 1, 1968·The Journal of Experimental Medicine·W H Lay, V Nussenzweig
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·W D BiggarR A Good
Nov 1, 1984·The Journal of Clinical Investigation·M BergerM M Frank
Oct 1, 1981·The Journal of Clinical Investigation·R B FickH Y Reynolds
Jul 1, 1958·A.M.A. Journal of Diseases of Children·H SHWACHMAN, L L KULCZYCKI
Apr 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·I MANDLB COHEN

❮ Previous
Next ❯

Citations

Oct 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J LundahlJ Hed
Feb 12, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Elaine HayesEmer P Reeves
Feb 22, 2012·Archivum Immunologiae Et Therapiae Experimentalis·David A BerginEmer P Reeves
Feb 16, 1991·Lancet·N G McElvaneyR G Crystal
Dec 1, 1990·FEMS Microbiology Immunology·A S Cross, N M Kelly
Dec 18, 2001·Advanced Drug Delivery Reviews·P Meers
Dec 16, 1997·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·B C Hilman
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·C J TaylorJ Karmel
Feb 3, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Gabriella BergaminiClaudio Sorio
Jul 18, 1996·The New England Journal of Medicine·B W Ramsey
Aug 20, 2002·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·André M CantinRichard Leduc
Feb 16, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mireille St-OngeMarc Pouliot
Oct 12, 2010·Pediatric Emergency Care·Chang-Teng Wu, Jing-Long Huang
Dec 1, 1991·Clinical and Experimental Immunology·H E FeuchtE Albert
Jan 1, 1991·Annals of the New York Academy of Sciences·S I RennardA B Thompson
Mar 1, 1994·Archives of Disease in Childhood·A J Henderson
Jan 1, 1994·Mediators of Inflammation·V Witko-Sarsat, B Descamps-Latscha
Mar 28, 1998·American Journal of Respiratory and Critical Care Medicine·M MikamiR A Stockley
Oct 6, 1999·American Journal of Respiratory and Critical Care Medicine·A M Cantin, D E Woods
Oct 1, 1991·The American Review of Respiratory Disease·J C WilliamsR D Krell
Aug 1, 1993·The American Review of Respiratory Disease·C B RobinsonC E Cross
Sep 1, 1993·The American Review of Respiratory Disease·A Buret, A W Cripps
Dec 1, 1993·The American Review of Respiratory Disease·C M O'ConnorM X Fitzgerald
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·G Döring
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·R WilsonA D Jackson
Dec 13, 2003·American Journal of Respiratory and Critical Care Medicine·Marie N BeckerScott H Randell
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Clifford C TaggartNoel G McElvaney
Sep 9, 2006·American Journal of Respiratory and Critical Care Medicine·Ali RoghanianJean-Michel Sallenave
Nov 22, 2002·American Journal of Respiratory Cell and Molecular Biology·André M CantinRichard Leduc
Nov 1, 1991·The Journal of Clinical Investigation·M B FasanoC E McCall
May 8, 2010·The Open Respiratory Medicine Journal·Derek J QuinnClifford C Taggart
Apr 5, 2012·Chest·Louise E Donnelly, Peter J Barnes
Oct 24, 2012·Molecular Immunology·James D Chalmers, Adam T Hill

❮ Previous
Next ❯

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