Complement regulatory protein CD46 induces autophagy against oxidative stress-mediated apoptosis in normal and asthmatic airway epithelium

Scientific Reports
Yi-Giien TsaiChing-Yuang Lin

Abstract

Autophagy plays a major role in defending against oxidative stress in respiratory epithelial cells. The complement regulatory protein CD46 can enhance autophagy and decrease local complement activation at sites of inflammation. This study investigated the mechanism by which CD46 protects against oxidative stress-mediated apoptosis in respiratory epithelium in asthmatic patients. Nasal mucosa samples were obtained from 60 adults with mild asthma who received turbinectomy and 30 controls. A decreased expression of CD46 and increased apoptosis were noted in the damaged nasal epithelium from the asthmatic patients. Primary epithelial cells cultured with Dermatophagoides pteronyssinus 2 showed decreased CD46 and increased cleaved CASPASE-3A expressions. Crosslinking CD46 mAb could induce the formation of autophagosomes and LC3-II expression in primary epithelial cells. CD46 engagement could induce autophagy against hydrogen peroxide-induced epithelial cell death, whereas the autophagy inhibitor 3-methyladenine decreased this effect. In addition, CD46 engagement decreased the expressions of PRO-IL-1β and NLRP3, enhanced the expression of scaffold protein GOPC, and diminished hydrogen peroxide-induced 8-OHdG, IL-1β and IL-6 production...Continue Reading

References

Sep 1, 1995·American Journal of Respiratory and Critical Care Medicine·S VarsanoD Ophir
Aug 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Fabio BucchieriDonna E Davies
Aug 25, 2004·Trends in Immunology·Rebecca C Riley-VargasJohn P Atkinson
Sep 7, 2004·Current Opinion in Allergy and Clinical Immunology·Hajime Takizawa
Aug 3, 2005·The Journal of Clinical Investigation·Istvan BoldoghSanjiv Sur
Aug 10, 2005·The Journal of Biological Chemistry·Kristina ElwardPhilippe Gasque
Nov 14, 2006·The Journal of Clinical Investigation·Anne L AstierDavid A Hafler
Oct 27, 2007·Molecular Immunology·L A TrouwP Gasque
May 17, 2008·American Journal of Respiratory Cell and Molecular Biology·Catherine M McDougallGarry M Walsh
Oct 20, 2009·Cell Host & Microbe·Pierre-Emmanuel JoubertMathias Faure
Nov 10, 2009·The Journal of Allergy and Clinical Immunology·Anne M FitzpatrickUNKNOWN National Institutes of Health/National Heart, Lung, and Blood Institute Severe Asthma Research Program
Jan 1, 2008·Current Molecular Pharmacology·P D Zalewski, R E Ruffin
Jan 21, 2010·Autophagy·Grégory MeiffrenMathias Faure
Apr 21, 2010·Expert Review of Clinical Immunology·Xun Zhang, Jörg Köhl
Sep 30, 2010·Antioxidants & Redox Signaling·Ju HuangJohn H Brumell
Dec 21, 2010·Current Allergy and Asthma Reports·Yves LaumonnierJörg Köhl
Dec 28, 2010·Biochemical and Biophysical Research Communications·Daisuke InoueMasayuki Yamamoto
Jan 14, 2011·The Journal of Biological Chemistry·James HarrisEd C Lavelle
May 17, 2011·PloS One·Sandra GrumelliCraig Gerard
Jul 15, 2011·The Journal of Allergy and Clinical Immunology·Chang XiaoDonna E Davies
Nov 2, 2011·The Journal of Allergy and Clinical Immunology·Audrey H PoonCatherine Laprise
Dec 31, 2011·The Journal of Allergy and Clinical Immunology·Sheena D BrownAnne M Fitzpatrick
Feb 7, 2012·Current Opinion in Allergy and Clinical Immunology·Amy Auerbach, Michelle L Hernandez
Mar 23, 2012·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Melda CelikOmer Kalayci
Apr 14, 2012·Autophagy·Audrey PoonQutayba Hamid
Jul 4, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yi-Giien TsaiChing-Yuang Lin
Nov 13, 2012·Current Opinion in Pulmonary Medicine·Soma S S K Jyothula, N Tony Eissa
Nov 28, 2012·Frontiers in Oncology·Sameera Kongara, Vassiliki Karantza
Feb 15, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
Mar 15, 2013·Allergy and Asthma Proceedings·Fabio CardinaleRiccardina Tesse
May 15, 2013·Molecular Immunology·Li ZuoKatherine S Salisbury
May 25, 2013·Free Radical Biology & Medicine·Matthew DodsonJianhua Zhang
May 28, 2013·Molecules and Cells·Stefan W RyterAugustine M K Choi
Sep 26, 2013·Nature Reviews. Immunology·Vojo DereticShizuo Akira
Oct 16, 2013·Internal Medicine·Jun ArayaKazuyoshi Kuwano
Feb 4, 2014·Antioxidants & Redox Signaling·Juliana Navarro-YepesRodrigo Franco
Mar 7, 2014·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Sameer Hasan Fatani
Apr 29, 2014·The Journal of Allergy and Clinical Immunology·Hortensia Moreno-Macias, Isabelle Romieu
May 9, 2014·Cell Biochemistry and Biophysics·Pradip K KamatNeetu Tyagi
Jul 6, 2014·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Lan JiangLi Zuo
Jul 30, 2014·The Journal of Allergy and Clinical Immunology·Yi-Giien TsaiChing-Yuang Lin

❮ Previous
Next ❯

Citations

Apr 5, 2020·European Journal of Immunology·Ursula SmoleWinfried F Pickl
Aug 6, 2020·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Kwangsik NhoUNKNOWN Alzheimer's Disease Neuroimaging Initiative
Jul 6, 2020·British Journal of Pharmacology·Ben C KingAnna M Blom
Jan 29, 2021·Microbiology and Molecular Biology Reviews : MMBR·Elisabet Bjanes, Victor Nizet
May 8, 2021·Journal of Cellular and Molecular Medicine·Jiapeng HuYunxiao Shang
May 19, 2021·Current Opinion in Immunology·M Kathryn Liszewski, John P Atkinson
Jul 3, 2021·International Journal of Molecular Sciences·Efthymia Theofani, Georgina Xanthou

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
biopsies
flow cytometry
confocal microscopy
ELISA
Assay
transfection
protein assay

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.