Complement regulatory protein expression by a human oligodendrocyte cell line: cytokine regulation and comparison with astrocytes

Immunology
P Gasque, B P Morgan

Abstract

Rat oligodendrocytes spontaneously activate complement (C) and lack the C inhibitor CD59. As a consequence, rat oligodendrocytes are susceptible to lysis by autologous C in vitro. Expression of C inhibitors on human oligodendrocytes in vitro and other human glia has yet to be well characterized. We have previously shown expression at the mRNA level of the membrane inhibitors CD59, decay-accelerating factor (DAF; CD55) and membrane cofactor protein (MCP; CD46) in human astrocytes. We here examine the expression of membrane and secreted C inhibitors by the oligodendrocyte cell line, HOG. HOG cells abundantly expressed CD59, assessed at protein and mRNA level, and expressed DAF and MCP, albeit at a lower level. Expression of all three inhibitors was enhanced by incubation with interferon-gamma or with phorbol ester (PMA). Complement receptor type 1 (CR1; CD35) was neither expressed constitutively nor induced by cytokines. HOG also constitutively secreted C1-inhibitor, S-protein and clusterin. Factor H was secreted only after stimulation with cytokines. C4b binding protein was expressed at a very low level and was detected only at the mRNA level by reverse transcriptase-polymerase chain reaction (RT-PCR). For comparison, astrocyte ...Continue Reading

References

Jun 1, 1992·Brain Research. Molecular Brain Research·D G Walker, P L McGeer
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J RogersP Ward
Mar 1, 1992·Journal of the Neurological Sciences·J ZajicekD A Compston
Sep 1, 1991·Immunology Today·M M Frank, L F Fries
Jan 1, 1989·Annual Review of Immunology·D M Lublin, J P Atkinson
Feb 1, 1982·The Journal of Experimental Medicine·J C CyongN K Day
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P VanguriM L Shin
Jan 1, 1995·Critical Reviews in Clinical Laboratory Sciences·B P Morgan
Jan 1, 1995·Journal of Neuropathology and Experimental Neurology·T KashimaA T Campagnoni
Jan 1, 1995·Annual Review of Immunology·J E Volanakis
Jan 1, 1996·Acta Neuropathologica·R VeerhuisP Eikelenboom

❮ Previous
Next ❯

Citations

Sep 23, 2000·Brain Research. Molecular Brain Research·R StrohmeyerJ Rogers
Apr 22, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C A SchmittW G Dippold
Aug 26, 2004·Journal of Neuroinflammation·Masato HosokawaPatrick L McGeer
Aug 10, 2000·Clinical and Experimental Immunology·O B SpillerB P Morgan
May 26, 1998·Journal of Neuroimmunology·N J ScoldingD A Compston
Sep 19, 2009·Neuromolecular Medicine·Trent M WoodruffStephen M Taylor
Oct 3, 2008·Journal of Neuroscience Research·Katrin FärberHelmut Kettenmann
Aug 29, 2013·Immunology·Laura PeferoenSandra Amor
Oct 2, 2015·Brain Research·Thomas ZeisNicole Schaeren-Wiemers
Jul 23, 2015·Journal of Biomedical Science·M Afzal KhanD C Broering
Jul 19, 2000·Biochimica Et Biophysica Acta·M R EmmerlingK Spiegel
Aug 8, 2009·Progress in Brain Research·V Ramaglia, F Baas
Mar 20, 2019·Frontiers in Immunology·Sarah M CarpaniniBryan Paul Morgan
Oct 27, 2016·Immunological Reviews·Ali Alawieh, Stephen Tomlinson
Sep 10, 2019·Frontiers in Psychiatry·Mélanie Druart, Corentin Le Magueresse
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S JunnikkalaS Meri
Feb 19, 2021·The FEBS Journal·Oluwaseun FatobaToshihide Yamashita
Mar 6, 2021·Schizophrenia Research·Daniela EnacheValeria Mondelli
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Lynn NazarethJenny Ekberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.