PMID: 9553648Apr 29, 1998Paper

Complement regulatory proteins on the sperm surface: relevance to sperm motility

American Journal of Reproductive Immunology : AJRI
H Jiang, S Pillai

Abstract

To determine whether complement regulatory proteins are present on human spermatozoa and whether antibodies to these proteins adversely affect sperm motility. Human sperm membrane proteins were solubilized and subjected to polyacrylamide gel electrophoresis followed by Western blot analysis against antibodies to complement component 1 inhibitor (C1-INH), decay-activating factor (DAF; CD55), membrane cofactor protein (MCP; CD46), and homologous restriction factor (HRF; CD59). Spermatozoa, obtained by a swim-up technique, were incubated in medium (control 1) and medium supplemented with antibodies to human albumin (control 2) and antibodies to these complement regulatory proteins. We used a computerized sperm motion analysis to determine the effect of these antibodies on sperm motion characteristics. Complement regulatory proteins such as C1-INH, CD55, CD46, and CD59 were found in the sperm extracts as shown by Western blot analysis. The heat-treated (56 degrees C, 30 min) IgG fraction of antibodies to these proteins significantly reduced sperm motility in general and other motion parameters. Addition of complement did not affect these results except in the antibodies to CD46 in which the reducing action was further amplified. Ou...Continue Reading

References

Apr 1, 1992·American Journal of Reproductive Immunology : AJRI·O A VanderpuyeJ A McIntyre
Jun 1, 1988·American Journal of Reproductive Immunology and Microbiology : AJRIM·S MathurH O Williamson
Nov 1, 1986·American Journal of Reproductive Immunology and Microbiology : AJRIM·S MathurH O Williamson
Mar 1, 1993·Biology of Reproduction·S E BozasI D Walker
Jun 1, 1994·Fertility and Sterility·R K Naz, A C Menge
Dec 31, 1997·American Journal of Reproductive Immunology : AJRI·H JiangH Zhang

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Citations

Oct 4, 2005·Springer Seminars in Immunopathology·M Kathryn LiszewskiJohn P Atkinson
Oct 31, 2009·Journal of Reproductive Immunology·Koji KoyamaShinji Komori
Mar 12, 2008·International Journal of Andrology·Dorota FiszerMaciej Kurpisz
Aug 25, 2004·Trends in Immunology·Rebecca C Riley-VargasJohn P Atkinson
Aug 19, 2005·Journal of Reproductive Immunology·A Hasegawa, K Koyama
Apr 22, 2016·Human Genomics·Chelsea L LoughnerShivalingappa K Swamynathan
Apr 26, 2005·The Journal of Clinical Investigation·Rebecca C Riley-VargasJohn P Atkinson
Nov 16, 2004·BJU International·Alistair B StewartAlan Cooper
Dec 31, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tamio FukushimaIkuo Horii
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xuebin QinJose A Halperin
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunita GulatiSanjay Ram
Nov 4, 2008·Scandinavian Journal of Urology and Nephrology·Alistair B StewartBashir A Lwaleed
Jul 12, 2002·Molecular Reproduction and Development·Rebecca C RileyJohn P Atkinson

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