PMID: 2121710Nov 1, 1990Paper

Complementarity of Bacillus subtilis 16S rRNA with sites of antibiotic-dependent ribosome stalling in cat and erm leaders

Journal of Bacteriology
E J RogersP S Lovett

Abstract

Inducible cat and erm genes are regulated by translational attenuation. In this regulatory model, gene activation results from chloramphenicol- or erythromycin-dependent stalling of a ribosome at a precise site in the leader region of cat or erm transcripts. The stalled ribosome is believed to destabilize a downstream region of RNA secondary structure that sequesters the ribosome-binding site for the cat or erm coding sequence. Here we show that the ribosome stall sites in cat and erm leader mRNAs, designated crb and erb, respectively, are largely complementary to an internal sequence in 16S rRNA of Bacillus subtilis. A tetracycline resistance gene that is likely regulated by translational attenuation also contains a sequence in its leader mRNA, trb, which is complementary to a sequence in 16S rRNA that overlaps with the crb and erb complements. An in vivo assay is described which is designed to test whether 16S rRNA of a translating ribosome can interact with the crb sequence in mRNA in an inducer-dependent reaction. The assay compares the growth rate of cells expressing crb-86 with the growth rate of cells lacking crb-86 in the presence of subinhibitory levels of inducers of cat-86, chloramphenicol, fluorothiamphenicol, amice...Continue Reading

References

Aug 1, 1990·Journal of Bacteriology·E J Rogers, P S Lovett
Jan 1, 1990·Journal of Bacteriology·P S Lovett
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Hui, H A de Boer
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Z AlexievaP S Lovett
Jan 1, 1984·Annual Review of Biochemistry·H F Noller
Jan 1, 1983·Methods in Enzymology·M J Zoller, M Smith
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D G Yansura, D J Henner

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Citations

Jul 2, 2011·FEMS Microbiology Letters·Nadya Andini, Kevin A Nash
Jun 1, 1996·Microbiological Reviews·P S Lovett, E J Rogers
Jul 1, 1991·Antimicrobial Agents and Chemotherapy·R Leclercq, P Courvalin

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