Complementary DNA cloning of rat spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids

Molecular Reproduction and Development
Hiroshi IidaYosaburo Shibata

Abstract

We used differential display in combination with complementary DNA (cDNA) cloning approach to isolate a novel rat gene designated as spetex-1, which had an open reading frame of 1,668-length nucleotides encoding a protein of 556 amino acids. Spetex-1 mRNA was highly expressed in testis, and weekly expressed in lung, intestine, and spleen. Spetex-1 expression in the rat testes was detected first at 3 weeks in postnatal development and continued to be detected up to adulthood. A search in the databases showed that the amino acid sequence of spetex-1 was 82% identical to that of its mouse homologue found in the databases. Both rat spetex-1 and the mouse homologue contained Ser-X (X = His, Arg, or Asn) repeats in the middle portion of the proteins. In situ hybridization revealed that spetex-1 mRNA was expressed in haploid spermatids of step 7-18 within the seminiferous epithelium. Immunohistochemical analysis with confocal laser-scanning microscopy demonstrated that spetex-1 protein was not expressed in spermatogonia, spermatocytes, and round spermatids in adult rat testis, but was specifically detected in the residual cytoplasm of elongate spermatids of step 15-18 as well as in residual bodies engulfed by Sertoli cells. We interpr...Continue Reading

References

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Citations

Feb 9, 2011·Molecular and Cellular Biology·Chamutal BornsteinVarda Rotter
May 27, 2009·PLoS Biology·Deanna M ChurchUNKNOWN Mouse Genome Sequencing Consortium
Sep 9, 2010·Zoological Science·Sayaka ShimasakiHiroshi Iida
Feb 14, 2014·Zoological Science·Sayoko OikiHiroshi Iida
Jul 12, 2011·Molecular Reproduction and Development·Hiroe TakiguchiHiroshi Iida
Sep 7, 2006·Molecular Reproduction and Development·Masamichi DoiguchiHiroshi Iida
Oct 11, 2007·Molecular Reproduction and Development·Emi MurayamaHiroshi Iida
Jun 11, 2005·Molecular Reproduction and Development·Takuya MatsuyamaHiroshi Iida
Dec 20, 2005·Molecular Reproduction and Development·Hiroshi IidaTetsuichiro Inai
Apr 6, 2006·Molecular Reproduction and Development·Hiroshi IidaTetsuichiro Inai

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