Complementary DNA libraries: an overview

Molecular Biotechnology
Shao-Yao Ying

Abstract

The generation of complete and full-length cDNA libraries for potential functional assays of specific gene sequences is essential for most molecules in biotechnology and biomedical research. The field of cDNA library generation has changed rapidly in the past 10 yr. This review presents an overview of the method available for the basic information of generating cDNA libraries, including the definition of the cDNA library, different kinds of cDNA libraries, difference between methods for cDNA library generation using conventional approaches and a novel strategy, and the quality of cDNA libraries. It is anticipated that the high-quality cDNA libraries so generated would facilitate studies involving genechips and the microarray, differential display, subtractive hybridization, gene cloning, and peptide library generation.

Citations

Oct 14, 2010·International Journal of Biological Sciences·Chang-Qing LiuYue-Hui Ma
Jan 18, 2011·Protist·Sarah E HamsherGary W Saunders
Feb 20, 2008·Current Opinion in Chemical Biology·Yoko YashirodaMinoru Yoshida
Feb 28, 2017·International Journal of Genomics·Nehir Ozdemir OzgenturkKemal Altunatmaz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.