Abstract
Monoclonal antibodies (mAbs) for therapeutic applications should be as similar to native human antibodies as possible to minimize their immunogenicity in patients. Several transgenic animal platforms are available for the generation of fully human mAbs. Attributes such as specificity, efficacy and Chemistry, Manufacturing and Controls (CMC) developability of antibodies against a specific target are typically established for antibodies obtained from one platform only. In this study, monoclonal antibodies (mAbs) cross-reactive against human and cynomolgus LAMP1 were derived from the human immunoglobulin transgenic TRIANNI mouse and OmniChicken® platforms and assessed for their specificity, sequence diversity, ability to bind to and internalize into tumor cells, expected immunogenicity and CMC developability. Our results show that the two platforms were complementary at providing a large diversity of mAbs with respect to epitope coverage and antibody sequence diversity. Furthermore, most antibodies originating from either platform exhibited good manufacturability characteristics.
References
Aug 7, 1975·Nature·G Köhler, C Milstein
Oct 1, 1990·Chemical & Pharmaceutical Bulletin·K AkasakiH Tsuji
Jun 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·A LipińskaA Krześlak
Aug 1, 1997·Hybridoma·K E KilpatrickG D McIntyre
Jan 14, 1998·International Journal of Cancer. Journal International Du Cancer·V SarafianS W Coninck
Dec 21, 2004·Nucleic Acids Research·Véronique GiudicelliMarie-Paule Lefranc
Nov 13, 2007·Journal of Immunological Methods·Thomas TillerHedda Wardemann
Jul 25, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sylvia JulienCyril Berthet
Sep 4, 2012·Journal of Clinical Immunology·Leslie P CousensAnne S De Groot
Jul 13, 2013·Blood·André CohnenCarsten Watzl
Aug 28, 2013·Current Drug Discovery Technologies·Larry L Green
May 23, 2014·Biochemical and Biophysical Research Communications·Akhil Kumar AgarwalRajiv D Kalraiya
May 1, 2011·Immunome Research·Timothy J MessittAnne S De Groot
Dec 4, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Marianne BrüggemannRoland Buelow
Dec 23, 2014·MAbs·Dawn M EckerHoward L Levine
Mar 31, 2016·PloS One·Dale O StarkieDaniel J Lightwood
Apr 24, 2016·Microscopy·Shelley Mettler IzquierdoWilliam Harriman
Sep 25, 2016·Biochemical and Biophysical Research Communications·Kazue TerasawaMiki Hara-Yokoyama
Oct 17, 2017·MAbs·Kathryn H ChingPhilip A Leighton
Jan 5, 2018·MAbs·Hélène Kaplon, Janice M Reichert
Feb 8, 2018·Journal of Experimental & Clinical Cancer Research : CR·Cécile Chalouni, Sophia Doll
Jun 29, 2018·Frontiers in Immunology·Philip A LeightonKathryn H Ching
Dec 14, 2018·MAbs·Yingda XuHongcheng Liu
Sep 24, 2019·Antibodies·Devendra BhandariRoger C Bridgman