Complementary expression of HIP, a cell-surface heparan sulfate binding protein, and perlecan at the human fetal-maternal interface

Biology of Reproduction
L H RohdeD D Carson

Abstract

The human hemochorial placenta is a structure formed by the invasion of cytotrophoblasts into the uterus. Previous studies from our laboratory have demonstrated a role for heparan sulfate proteoglycans (HSPGs) and their binding proteins in interactions between human trophoblastic and uterine cell lines in vitro. In this study, expression of both mRNA and protein of a novel, cell surface, heparin/heparan sulfate interacting protein (HIP), by human trophoblastic cell lines-i.e., JAR, JEG, and BeWo-and by human cytotrophoblast was examined throughout gestation. Immunohistochemistry of the human fetal-maternal interface demonstrated abundant HIP expression in cytotrophoblast cells, with lesser staining in syncytiotrophoblast and little or no staining in surrounding stromal or decidual cells. Staining with antibodies to the basement membrane HSPG, perlecan, demonstrated a pattern of staining complementary to that of HIP. Cytotrophoblasts in the uterine stroma, not affiliated with attached villi, displayed a less intense deposition of perlecan. In vitro binding studies of 125I-perlecan to a 17-amino acid synthetic peptide sequence of HIP, which has a high affinity and specificity for heparin/heparan sulfate, indicates that perlecan b...Continue Reading

Citations

Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M Slater, C R Murphy
Jun 7, 2002·Journal of Virology·D G Hemmings, L J Guilbert
Nov 3, 2012·Disease Models & Mechanisms·Dylan T JonesKairbaan M Hodivala-Dilke
Sep 4, 2007·Seminars in Cell & Developmental Biology·Catherine B Kirn-SafranDaniel D Carson
Nov 5, 2008·Journal of Cellular Biochemistry·Sonia D'SouzaDaniel D Carson
Jul 7, 2000·Developmental Biology·D D CarsonK Yoshinaga
Jun 23, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lynne ThadikkaranJean-Daniel Tissot
Sep 5, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Mary C Farach-CarsonDaniel D Carson
Aug 16, 2003·Differentiation; Research in Biological Diversity·Stephanie A MillerCatherine B Kirn-Safran
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Juliana L DreyfussHelena B Nader
Jan 23, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Catherine B Kirn-SafranDaniel D Carson
Apr 21, 2012·Gynecologic and Obstetric Investigation·A ChuiJ M Said
Jan 5, 2020·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Murat AkbasHabib Ozdemir
Sep 17, 1998·The Journal of Biological Chemistry·D E HokeD D Carson
Dec 22, 1999·Biochemical and Biophysical Research Communications·P TapanadechoponeJ R Couchman
Jun 30, 2000·Seminars in Cell & Developmental Biology·S J Kimber, C Spanswick

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