Complementary methods for SARS-CoV-2 diagnosis in times of material shortage.

Scientific Reports
Thaisa Lucas SandriAndrea Kreidenweiss

Abstract

The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2-particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources.

References

Apr 14, 2020·Annals of Internal Medicine·Matthew P ChengCedric P Yansouni
Aug 9, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Erick Gustavo DorlassDanielle Bruna Leal Oliveira
Feb 26, 2021·The Journal of Applied Laboratory Medicine·Bijal A ParikhNeil W Anderson
Mar 30, 2020·MedRxiv : the Preprint Server for Health Sciences·Ignazio Ciufolini, A. Paolozzi

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Software Mentioned

GraphPad Prism
uMELT Quartz
LightCycler 480

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