Complementary roles of KCa3.1 channels and β1-integrin during alveolar epithelial repair

Respiratory Research
Alban GiraultEmmanuelle Brochiero

Abstract

Extensive alveolar epithelial injury and remodelling is a common feature of acute lung injury and acute respiratory distress syndrome (ARDS) and it has been established that epithelial regeneration, and secondary lung oedema resorption, is crucial for ARDS resolution. Much evidence indicates that K(+) channels are regulating epithelial repair processes; however, involvement of the KCa3.1 channels in alveolar repair has never been investigated before. Wound-healing assays demonstrated that the repair rates were increased in primary rat alveolar cell monolayers grown on a fibronectin matrix compared to non-coated supports, whereas an anti-β1-integrin antibody reduced it. KCa3.1 inhibition/silencing impaired the fibronectin-stimulated wound-healing rates, as well as cell migration and proliferation, but had no effect in the absence of coating. We then evaluated a putative relationship between KCa3.1 channel and the migratory machinery protein β1-integrin, which is activated by fibronectin. Co-immunoprecipitation and immunofluorescence experiments indicated a link between the two proteins and revealed their cellular co-distribution. In addition, we demonstrated that KCa3.1 channel and β1-integrin membrane expressions were increased...Continue Reading

References

Jan 1, 1974·Preparative Biochemistry·P H Cooper, D R Stanworth
Nov 1, 1983·The Journal of Cell Biology·T NishidaR Manabe
Aug 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D Sheppard
Nov 1, 1996·American Journal of Respiratory Cell and Molecular Biology·A L HerardE Puchelle
Jul 10, 1999·Pflügers Archiv : European journal of physiology·A SchwabP C Dartsch
Feb 9, 2000·American Journal of Physiology. Cell Physiology·J Y WangJ X Yuan
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
Feb 5, 2002·The Journal of Physiology·Elizabeth A Cowley, Paul Linsdell
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Jaladanki N RaoJian-Ying Wang
Apr 10, 2002·Cell Biochemistry and Biophysics·Michael J DavisEmily Wilson
Aug 9, 2003·Critical Care Medicine·David W ShimabukuroMichael A Gropper
Jan 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudie LeroyEmmanuelle Brochiero
Apr 27, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Emmanuelle BrochieroRyszard Grygorczyk
Nov 12, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jaladanki N RaoJian-Ying Wang
Aug 1, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·André DagenaisYves Berthiaume
Aug 8, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudie LeroyEmmanuelle Brochiero
Oct 27, 2006·Trends in Cell Biology·Annarosa Arcangeli, Andrea Becchetti
Nov 7, 2006·Clinics in Chest Medicine·G R Scott Budinger, Jacob I Sznajder
Jun 21, 2007·Annual Review of Biochemistry·Kartik Venkatachalam, Craig Montell
Jul 17, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nguyen Thu Ngan TrinhEmmanuelle Brochiero
Nov 6, 2007·Molecular and Cellular Biochemistry·William Ka Kei WuChi Hin Cho
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gregory W deHartDean Sheppard
Jul 11, 2008·Journal of Cellular Physiology·Jian-Feng WeiShan Ping Yu
Aug 8, 2008·Proceedings of the American Thoracic Society·Christelle CorauxPhilippe Birembaut
Sep 2, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nguyen Thu Ngan TrinhEmmanuelle Brochiero
Dec 5, 2008·American Journal of Physiology. Cell Physiology·Hélène KleinRémy Sauvé
Apr 7, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lynn M Crosby, Christopher M Waters
Jun 4, 2010·Biochemical and Biophysical Research Communications·Marie PotierFabrice Pierre
Jun 17, 2010·Advances in Experimental Medicine and Biology·Sara CabodiPaola Defilippi
Jan 8, 2011·Critical Care : the Official Journal of the Critical Care Forum·Maina M B MoralesMarisa Dolhnikoff
Jun 3, 2011·World Journal of Gastroenterology : WJG·Masahiro Iizuka, Shiho Konno
Jul 29, 2011·The Journal of Biological Chemistry·Melissa MillerMichael X Zhu
Aug 30, 2011·The Journal of Allergy and Clinical Immunology·Glenn CrusePeter Bradding
Sep 13, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Emilie MailléEmmanuelle Brochiero
Sep 29, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andreas SchwingshacklChristopher M Waters

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Citations

Jun 30, 2016·Scientific Reports·Linda Sevelsted MøllerJonel Trebicka
Jun 27, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Takuya TsukaharaShinichi Kato
Aug 3, 2018·Biological & Pharmaceutical Bulletin·Susumu Ohya, Hiroaki Kito
Aug 5, 2017·Journal of Cellular Physiology·Ekaterina Pchelintseva, Mustafa B A Djamgoz
Apr 20, 2016·PloS One·Hélène KleinRémy Sauvé
Aug 16, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andreas Schwingshackl
Oct 10, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ju Jing TanRyszard Grygorczyk
Apr 5, 2019·Mediators of Inflammation·Franziska M KonradJörg Reutershan
Nov 15, 2019·Physiological Reports·Mélissa Aubin VegaEmmanuelle Brochiero
Jan 7, 2017·American Journal of Respiratory Cell and Molecular Biology·Louise OrganKen J Snibson
Aug 8, 2021·International Journal of Molecular Sciences·Nadezhda BarvitenkoAntonella Pantaleo

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Methods Mentioned

BETA
transfection
co-immunoprecipitation
PCR
immunoprecipitation
co-immunoprecipation

Software Mentioned

GraphPad Prism
GraphPad
AxioVision
Image Lab
Metafluor
ImageJ
Metafluor Analysis
ImageQuant
SPSS Statistics

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