PMID: 9437431Jan 23, 1998Paper

Complementary sets of noncanonical base pairs mediate RNA helix packing in the group I intron active site

Nature Structural Biology
S A StrobelE Moncoeur

Abstract

Helix packing is critical for RNA tertiary structure formation, although the rules for helix-helix association within structured RNAs are largely unknown. Docking of the substrate helix into the active site of the Tetrahymena group I ribozyme provides a model system to study this question. Using a novel chemogenetic method to analyze RNA structure in atomic detail, we report that complementary sets of noncanonical base pairs (a G.U wobble pair and two consecutively stacked sheared A.A pairs) create an RNA helix packing motif that is essential for 5'-splice site selection in the group I intron. This is likely to be a general motif for helix-helix interaction within the tertiary structures of many large RNAs.

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Citations

Dec 10, 1998·Biopolymers·S A Strobel
Apr 11, 2001·Biopolymers·S J SchroederD H Turner
Jun 1, 2005·Current Opinion in Structural Biology·Sarah A Woodson
Jul 24, 2003·Journal of Molecular Biology·Ivelitza Garcia, Kevin M Weeks
Aug 9, 2002·RNA·Matthieu G Gagnon, Sergey V Steinberg
Aug 24, 2013·Inorganic Chemistry·Anna J McConnellJacqueline K Barton
Dec 10, 1998·Nature Structural Biology·A A SzewczakS A Strobel
May 16, 2006·Nature Structural & Molecular Biology·Joshua A JansenJuliane K Soukup
Oct 3, 2002·The EMBO Journal·Christina WaldsichRenée Schroeder
Jan 6, 2009·Journal of Biochemistry·Yoshiya IkawaTan Inoue
Feb 28, 2002·Nucleic Acids Research·Vanita D SoodRichard A Collins
Mar 9, 2002·Nucleic Acids Research·Sean P Ryder, Scott A Strobel
Jun 11, 2004·Annual Review of Biochemistry·Gerald F Joyce
Nov 18, 2004·RNA·Peter L AdamsScott A Strobel
Aug 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roshan M Kumar, Gerald F Joyce
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A SzewczakS A Strobel
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·L JaegerG F Joyce
Mar 12, 2010·New Biotechnology·Tonje Fiskaa, Asa B Birgisdottir
Jul 7, 2007·Journal of Molecular Biology·Mary R StahleyScott A Strobel
Sep 4, 2007·Journal of Molecular Biology·Richard A LeaseSarah A Woodson
May 5, 2004·Journal of Molecular Biology·Prashanth RanganSarah A Woodson
May 16, 2006·Current Opinion in Structural Biology·Mary R Stahley, Scott A Strobel
Jun 11, 1999·Current Opinion in Structural Biology·S A Strobel
Aug 31, 2000·Annual Review of Biochemistry·E A Doherty, J A Doudna
Apr 3, 2002·Chemistry & Biology·Kathleen E McGinness, Gerald F Joyce
Mar 13, 1999·Chemistry & Biology·S A Strobel, L Ortoleva-Donnelly
Jul 10, 2001·Annual Review of Biophysics and Biomolecular Structure·E A Doherty, J A Doudna
Oct 9, 2014·Annual Review of Pharmacology and Toxicology·Daniel J Urban, Bryan L Roth
Jun 5, 2013·Journal of Biomolecular Structure & Dynamics·Ol'ha O Brovarets'Dmytro M Hovorun
Jul 25, 1998·Journal of Molecular Biology·J Pan, S A Woodson

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