Complementary use of bioluminescence imaging and contrast-enhanced micro-computed tomography in an orthotopic brain tumor model

Molecular Imaging
Sanaz YahyanejadMarc Vooijs

Abstract

Small animal models are crucial to link molecular discoveries and implementation of clinically relevant therapeutics in oncology. Using these models requires noninvasive imaging techniques to monitor disease progression and therapy response. Micro-computed tomography (CT) is less studied for the in vivo monitoring of murine intracranial tumors and traditionally suffers from poor soft tissue contrast, whereas bioluminescence imaging (BLI) is known for its sensitivity but is not frequently employed for quantifying tumor volume. A widely used orthotopic glioblastoma multiforme (GBM) tumor model was applied in nude mice, and tumor growth was evaluated by BLI and contrast-enhanced microCT imaging. A strong correlation was observed between CT volume and BLI-integrated intensity (Pearson coefficient (r)  =  .85, p  =  .0002). Repeated contouring of contrast-enhanced microCT-delineated tumor volumes achieved an intraobserver average pairwise overlap ratio of 0.84 and an average tumor volume coefficient of variance of 0.11. MicroCT-delineated tumor size was found to correlate with tumor size obtained via histologic analysis (Pearson coefficient (r)  =  .88, p  =  .005). We conclude that BLI intensity can be used to derive tumor volume b...Continue Reading

References

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Citations

Jul 6, 2018·The British Journal of Radiology·Brent van der HeydenFrank Verhaegen
Sep 28, 2018·The British Journal of Radiology·Lucas PersoonFrank Verhaegen
Nov 23, 2018·The British Journal of Radiology·Venus Sosa IglesiasMarc Vooijs
May 9, 2015·Journal of Neuro-oncology·Patrick V GrantonMarc A Vooijs
Feb 15, 2017·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Sara RapicSigrid Stroobants

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Methods Mentioned

BETA
fluorescence imaging
imaging techniques
imaging technique
X-ray
xenografts
bioluminescence imaging
xenograft

Software Mentioned

Matlab
Optix OptiView
OsiriX
ImageJ

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