Complementary use of MALDI and ESI for the HPLC-MS/MS analysis of DNA-binding proteins

Analytical Chemistry
Martha D Stapels, Douglas F Barofsky

Abstract

Proteins from Escherichia coli were isolated based on their ability to bind DNA and digested in-solution with trypsin; the resulting peptides were separated using HPLC and subsequently analyzed using MALDI TOF/TOF and ESI Q-TOF instruments. Various properties of the peptides observed with the two ionization techniques were compared taking into account the differences between the mass analyzers. This empirical analysis of a data set containing hundreds of peptides and thousands of individual amino acids supports some of the currently held notions regarding the complementary nature of the two ionization processes. Specifically, ESI tends to favor the identification of hydrophobic peptides whereas MALDI tends to lead to the identification of basic and aromatic species. Findings from the present study suggest that ESI and MALDI may be complementary due to the biases of the two ionization techniques for certain classes of amino acids. From a practical standpoint, these biases indicate that, for the present at least, analyses must be performed on both types of instruments in order to gain the most information possible out of a given set of samples in a proteomics study.

References

May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 8, 1999·Journal of the American Society for Mass Spectrometry·M T Davis, T D Lee
Feb 3, 2000·Analytical Chemistry·H OrsnesK K Murray
Jul 3, 2003·Journal of the American Society for Mass Spectrometry·Ruedi Aebersold
Aug 22, 2003·Molecular & Cellular Proteomics : MCP·Alma L Burlingame
Sep 5, 2003·Journal of the American Society for Mass Spectrometry·Wanda M BodnarM Arthur Moseley

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Citations

Apr 18, 2013·Journal of the American Society for Mass Spectrometry·Andrew R Korte, Young Jin Lee
Jul 25, 2013·Experimental Eye Research·Nadine von Thun Und Hohenstein-BlaulFranz H Grus
Jul 3, 2007·Journal of the American Society for Mass Spectrometry·Sarah Trimpin, Max L Deinzer
May 13, 2006·Analytical Chemistry·Hilda HernandezI Jonathan Amster
Mar 3, 2006·Virology Journal·Jennifer D YoderDennis E Hruby
Jan 1, 2013·Toxicology and Applied Pharmacology·Kamlesh ShrivasMichail A Alterman
Mar 24, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tomás VaisarBryan J Prazen
Nov 17, 2009·Journal of Microbiological Methods·Ali R VaezzadehDenis F Hochstrasser
Nov 14, 2015·Journal of the American Chemical Society·Jong Wha LeeHugh I Kim
Aug 7, 2012·Journal of Neurochemistry·Elena V RomanovaJoshua M Gulley
May 18, 2013·Experimental Parasitology·Nurulhasanah OthmanRahmah Noordin
Sep 5, 2013·Free Radical Biology & Medicine·André M N SilvaPedro Domingues
Aug 26, 2014·Food Chemistry·Marta GallegoFidel Toldrá
Mar 19, 2016·Proteomics·Benjamin RuprechtBernhard Kuster
Nov 4, 2005·Nature·Stephen J GiovannoniDouglas F Barofsky
Jun 7, 2018·Rapid Communications in Mass Spectrometry : RCM·Nick Degraan-WeberJames P Reilly
Mar 8, 2005·Journal of Mass Spectrometry : JMS
Sep 24, 2005·Rapid Communications in Mass Spectrometry : RCM·Melkamu GetieReinhard H H Neubert
Jul 19, 2016·Scientific Reports·Sebastian FunkeFranz H Grus
May 8, 2018·Phytochemical Analysis : PCA·Hossam Al-SuodBogusław Buszewski
Oct 1, 2005·Comprehensive Reviews in Food Science and Food Safety·Wenyi Wang, Elvira Gonzalez De Mejia
Jul 25, 2007·Chemical Reviews·Gert Lubec, Leila Afjehi-Sadat
Jul 26, 2007·Chemical Reviews·Marjorie L FournierMichael P Washburn
Aug 9, 2007·Chemical Reviews·Anna E Speers, Christine C Wu
Feb 3, 2007·Journal of Proteome Research·Scott A SmithZheng Ouyang

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