Complementation cell lines for viral vectors to be used in gene therapy.

Cytotechnology
M Mehtali

Abstract

Viral vectors provide a highly efficient method for the transfer of foreign genes into a variety of quiescent or dividing eukaryotic cells from many animal origins. While recombinant vectors derived from an increasing number of mammalian viruses (herpes simplex virus, autonomous and non-autonomous parvoviruses, poxviruses, retroviruses, adenoviruses available today, vectors based on murine retroviruses and human adenoviruses constitute preferential candidates for the delivery of marker or therapeutic genes into human somatic cells. The availability of such vectors has made possible the recent transition of human gene therapy from laboratory benches to clinical settings. Most current recombinant vectors have been generated by deleting essential viral genes in order to make space available for the introduction of passenger genes. Such vectors are therefore unable to replicate in the absence of these critical gene products and their production relies on the development of stable complementation cell lines providingin trans the missing viral functions. Although complementation (or packaging) cell lines are available for both adenovirus and retrovirus vectors, their respective drawbacks still limit their use to research applications...Continue Reading

References

Mar 1, 1978·The Journal of Experimental Medicine·R M BartholomewH J Müller-Eberhard
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Jan 1, 1992·Biotechnology·F L Graham, L Prevec
Oct 1, 1992·The Journal of Experimental Medicine·R E DonahueR Moen
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L RaoE White
May 8, 1992·Science·W F Anderson
Jun 11, 1992·Nature·A D Miller
Jan 1, 1990·Human Gene Therapy·A D Miller
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E White, R Cipriani
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P A HantzopoulosE Gilboa
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O Danos, R C Mulligan
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S F YuE Gilboa
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·G KetnerP Hieter
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A J BettF L Graham
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ M Wilson
Jul 1, 1994·Journal of Virology·C A Deminie, M Emerman
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·D G MillerA D Miller
Jan 1, 1993·Methods in Enzymology·A D MillerC M Lynch
May 14, 1993·Science·R C Mulligan
Jul 30, 1994·Breast Cancer : the Journal of the Japanese Breast Cancer Society·T WadaM Yasutomi

❮ Previous
Next ❯

Citations

Jun 15, 1999·Trends in Pharmacological Sciences·D L Ennist

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.