Mar 2, 2014

Complementation of a temperature sensitive Escherichia coli rpoD mutation using Lactobacillus sigma factors

BioRxiv : the Preprint Server for Biology
James Winkler, Katy Kao


Housekeeping sigma factors in the σ 70 family, as components of the RNA polymerase holoenzyme, are responsible for regulating transcription of genes related to vegetative growth. While these factors are well understood in model organisms such as Escherichia coli and Bacillus subtilis , little experimental work has focused on the sigma factors in members of the Lactobacillus genus such as Lactobacillus brevis and Lactobacillus plantarum . This study evaluates the ability of putative σ 70 proteins from L. brevis (![Graphic][1]</img>) and L. plantarum (![Graphic][2]</img>) to complement a temperature sensitive mutation in the E. coli 285c σ 70 protein. This report is the first to show that these heterologous sigma factors were capable of restoring the viability of E. coli 285c for growth at 40-43.5 ° C , indicating the ![Graphic][3]</img> and ![Graphic][4]</img> are capable of initiating transcription in a complex with the E. coli 285c RNA polymerase. These heterologous sigma factors may therefore be useful for improving biochemical knowledge of the sigma factor family or for use in the expression of hetereologous genomic libraries. [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3....Continue Reading

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Mentioned in this Paper

Transcriptional Regulation
DNA-Directed RNA Polymerase
RNA Polymerase Assembly Pathway
Transcription, Genetic
Lactobacillus brevis
Developmental Vegetative Growth
Bacillus subtilis

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