Mar 2, 2014

Complementation of a temperature sensitive Escherichia coli rpoD mutation using Lactobacillus sigma factors

BioRxiv : the Preprint Server for Biology
James Winkler, Katy Kao

Abstract

Housekeeping sigma factors in the σ 70 family, as components of the RNA polymerase holoenzyme, are responsible for regulating transcription of genes related to vegetative growth. While these factors are well understood in model organisms such as Escherichia coli and Bacillus subtilis , little experimental work has focused on the sigma factors in members of the Lactobacillus genus such as Lactobacillus brevis and Lactobacillus plantarum . This study evaluates the ability of putative σ 70 proteins from L. brevis (![Graphic][1]</img>) and L. plantarum (![Graphic][2]</img>) to complement a temperature sensitive mutation in the E. coli 285c σ 70 protein. This report is the first to show that these heterologous sigma factors were capable of restoring the viability of E. coli 285c for growth at 40-43.5 ° C , indicating the ![Graphic][3]</img> and ![Graphic][4]</img> are capable of initiating transcription in a complex with the E. coli 285c RNA polymerase. These heterologous sigma factors may therefore be useful for improving biochemical knowledge of the sigma factor family or for use in the expression of hetereologous genomic libraries. [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3....Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Study
Transcriptional Regulation
Genome
Genes
DNA-Directed RNA Polymerase
RNA Polymerase Assembly Pathway
Transcription, Genetic
Lactobacillus brevis
Developmental Vegetative Growth
Bacillus subtilis

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.