PMID: 7527173Sep 1, 1994Paper

Complementation of human immunodeficiency virus type 1 vif mutants in some CD4+ T-cell lines

Virus Research
M Hevey, L A Donehower

Abstract

The viral infectivity factor gene, vif of human immunodeficiency virus type 1 (HIV-1), is required for full infectivity in most T-cell lines. The replication kinetics exhibited by these mutants has been shown to be cell type-dependent. In H9 cells as well as primary lymphocytes, vif mutants are incapable of establishing infection. This has led to classification of these cell types as non-permissive for vif mutant replication. The T-cell lines Sup T1 and C8166 are able to replicate the vif mutant virus, leading to their classification as permissive for vif mutant replication. In this study, four cell lines (Sup T1, C8166, Molt 4 Clone 8, and A3.01) were tested for their ability to replicate vif mutant virus derived from two different strains of HIV-1 (HXB2 and NL4-3) that had been passaged on various cell lines. Although the kinetics of initial infection was delayed in all cells, by the second passage of vif mutant virus on Sup T1 or Molt 4 cells the kinetics of replication were identical to wild type virus. In contrast, mutant virus displayed delayed replication kinetics in C8166 and A3.01 cells in both initial and subsequent passages. In addition, the levels of viral DNA in infected Sup T1 cells were similar for delta vif and ...Continue Reading

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Citations

Nov 29, 2005·Natural Product Reports·Muhammad SaleemYong Sup Lee
Aug 3, 2005·AIDS Research and Human Retroviruses·Kristine M RoseDavid Kabat
Feb 23, 2000·Journal of Virology·U ChatterjiJ H Elder
Dec 7, 2013·Journal of Viral Hepatitis·E M Janahi, M J McGarvey
Apr 8, 2015·European Journal of Medicinal Chemistry·Ting PanChuan Bai
May 25, 2006·International Journal of Hematology·Elana S Ehrlich, Xiao-Fang Yu
Jul 27, 2006·International Journal of Hematology·Andrew M L Lever, Kuan-Teh Jeang
Apr 11, 2012·World Journal of Hepatology·Megan CraneSharon R Lewin

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