Complementation of M gene mutants of vesicular stomatitis virus by plasmid-derived M protein converts spherical extracellular particles into native bullet shapes

Virology
Douglas S LylesW G Jerome

Abstract

The matrix (M) protein of vesicular stomatitis virus (VSV) binds the nucleocapsid to the cytoplasmic surface of the host plasma membrane during virus assembly by budding. It also condenses the nucleocapsid into a tightly coiled nucleocapsid-M protein complex that appears to give the virion its bullet-like shape. As described here, temperature-sensitive (ts) M mutants produced two classes of membrane-containing extracellular particles at the nonpermissive temperature. These could be distinguished by sedimentation in sucrose gradients and by electron microscopy. One class contained nucleocapsids and envelope glycoprotein, but very little M protein. The other class was devoid of nucleocapsids. Most of these particles were spherical or pleiomorphic in shape as determined by electron microscopy. Expression of wild-type (wt) M protein from plasmid DNA using the vaccinia/T7 virus system did not enhance the incorporation of nucleocapsids into extracellular particles from cells coinfected with the ts M mutants but did enhance the incorporation of M protein into these particles. Electron microscopy showed that wt M protein served to impart the bullet-like shape typical of VSV virions to what would otherwise be spherical or pleiomorphic v...Continue Reading

Citations

Apr 15, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jean-Simon DialloJohn C Bell
Jun 18, 2002·The EMBO Journal·Martin GaudierMarcel Knossow
Sep 11, 2009·Journal of Virology·Ewan F DunnJohn H Connor
Jan 1, 2012·Uirusu·Akira Nishizono, Kentaro Yamada
Mar 5, 2013·Médecine sciences : M/S·Valérie JanelleAlain Lamarre
May 9, 2012·Science China. Life Sciences·Ming Luo
Nov 30, 2004·Virus Research·Himangi R JayakarMichael A Whitt

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