Complementation of vaccinia virus deleted of the E3L gene by mutants of E3L

Virology
T ShorsB L Jacobs

Abstract

Vaccinia virus devoid of its E3L gene is sensitive to treatment of RK-13 cells with interferon-alpha and fails to replicate or form plaques in HeLa cells. In order to determine function of the E3L gene, vaccinia virus recombinants were constructed by inserting mutant E3L genes or a gene coding for an alternative dsRNA-binding protein into virus deleted of its wild type E3L gene. Those viruses that expressed proteins that retained dsRNA binding activity were resistant to the effects of interferon in RK-13 cells and could replicate in HeLa cells. Recombinant viruses that expressed E3L mutant proteins which were unable to bind to dsRNA were interferon sensitive in RK-13 cells and could not replicate in HeLa cells. In addition, a virus that expressed a mutant E3L protein previously characterized as having a low binding affinity for dsRNA exhibited an intermediate phenotype: it was interferon resistant in RK-13 cells but could not replicate in HeLa cells. This work suggests that the E3L gene of vaccinia virus functions primarily as a dsRNA-binding protein in allowing resistance to interferon and in promoting replication in HeLa cells.

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Citations

Oct 21, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jeffrey O LanglandBertram L Jacobs
Sep 1, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cyril X GeorgeCharles E Samuel
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Sep 22, 2006·Journal of Virology·Morgan HakkiAdam P Geballe
Jan 19, 2002·Journal of Virology·O P ZhirnovH-D Klenk
May 28, 2005·Journal of Virology·Morgan Hakki, Adam P Geballe
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·M A GarcíaM Esteban
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·C F BaslerP Palese
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yang-Gyun KimAlexander Rich
Sep 2, 1998·Current Opinion in Immunology·M Barry, G McFadden
Apr 25, 2007·Biochimie·M A GarcíaM Esteban
Jul 10, 2013·Cellular Microbiology·Jessica TsalikisStephen E Girardin
Apr 23, 2013·Virology·Craig J BierleAdam P Geballe
Jun 23, 2004·Virology·Jeffrey O Langland, Bertram L Jacobs
Jul 8, 2008·PLoS Pathogens·Susana GuerraMariano Esteban
Aug 19, 2007·Cytokine & Growth Factor Reviews·Michael P Gantier, Bryan R G Williams
Jul 1, 2008·Advances in Virus Research·Steven J WerdenGrant McFadden
May 25, 2011·Virus Research·Jana LiskovaZora Melkova
Aug 9, 2017·Viruses·Daniel Brian NicholsJessica Cottrell
Aug 8, 2015·Journal of Virology·William D ArndtBertram L Jacobs
Dec 19, 2002·International Reviews of Immunology·Christopher F Basler, Adolfo García-Sastre

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