PMID: 6987059Feb 1, 1980Paper

Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli

European Journal of Biochemistry
B LaineM Cohen-Solal

Abstract

The DNA-binding protein HU from Escherichia coli is a heterodimer constituted of two polypeptide chains termed HU-1 and HU-2, of 90 residues each. Their primary structures were established from structural data obtained from tryptic peptides of each monomer in addition to the structural data provided by the automated Edman degradation of the dimer and by peptides derived from cleavage of the dimer with trypsin, chymotrypsin, V8 staphylococcal protease and dilute acid. The results presented in this paper confirm the amino-terminal and carboxy-terminal sequences of the dimer HU reported previously [Laine et al. (1978) FEBS Lett. 89, 116--120]. The amino acid sequences of proteins HU-1 and HU-2 are identical to those of proteins NS-1 and NS-2 respectively, determined independently by Mende et al. [FEBS Lett. (1978) 96, 395--398]. The amino acid sequences of proteins HU-1 and HU-2 are closely related but differ by 28 residues. These proteins are characterized by their high content of hydrophobic residues represented mostly by alanine. In both proteins, half of the basic residues are scattered along the polypeptide chain and the remainder is found within two short sequences located in the carboxy-terminal part of the molecule. No seq...Continue Reading

References

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Citations

Sep 1, 1987·Molecular & General Genetics : MGG·Y KanoF Imamoto
Jan 1, 1985·Molecular & General Genetics : MGG·Y KanoF Imamoto
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N E Dixon, A Kornberg
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B ToussaintP M Vignais
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L Wang, X Q Liu
Mar 11, 1985·Nucleic Acids Research·B MignotteJ C Mounolou
Mar 15, 1983·European Journal of Biochemistry·B LaineP Sautière
Mar 1, 1985·European Journal of Biochemistry·H KhanakaJ Guillaume
Dec 15, 1986·European Journal of Biochemistry·B LaineP Sautiere
Nov 19, 2013·PLoS Genetics·Rudra P SahaRasika M Harshey
Jan 21, 2010·Biophysical Journal·Jiahn-Haur LiaoShih-Hsiung Wu
May 14, 1982·Biochemical and Biophysical Research Communications·B LaineG Biserte
Jan 1, 1990·Molecular & General Genetics : MGG·T OguraS Hiraga
May 18, 1999·Biochemical and Biophysical Research Communications·H HigashibataT Imanaka
Oct 1, 1988·Journal of Bacteriology·M DürrenbergerE Kellenberger
Apr 1, 1990·Biochimie·N GoshimaF Imamoto
Sep 1, 1987·Microbiological Reviews·K Drlica, J Rouviere-Yaniv
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Jun 15, 1984·Biochemical and Biophysical Research Communications·H KhanakaJ Guillaume
May 5, 1990·Journal of Molecular Biology·K KohnoF Imamoto

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